Tnfα antibody

SK-N-LP/luciferase cells were seeded in a 96-well plate (around 4000 cells/well). After overnight culture, SK-N-LP/luciferase cells were pre-treated with DMSO, TNFα antibody (Sigma-Aldrich, St. Louis, USA), caspase 1 (Biovision, San Diego, USA), and pan-caspase (Calbiochem, Darmstad, Germany) inhibitors for 2 h, respectively. The viability of neuroblastoma with Salmonella or macrophages supernatant treatment was observed by XTT kit (Roche, Mannheim, Germany) for three continuous days or after 2 days, respectively. The optical density was measured using a microplate reader at wavelength 450 nm.

Glycerin, Absolute Ethanol, Sodium Chloride, Bovine Serum Albumin (BSA), L-Alanine, D-Biotin, (3-Aminopropyl) trimethoxy silane (APTMS), (3-Mercaptopropyl) trimethoxy silane (MPTES), Interleukin-6 (IL-6), IL-6 antibody, tumor necrosis factor alpha (TNF-α), and TNF-α antibody were purchased from Sigma-Aldrich, France. Phosphate-buffered saline (PBS), Dulbecco’s Modified Eagle Medium (DMEM), N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (HEPES), Fetal bovine serum (FBS), Glutamine, and Trypsin/EDTA solutions were purchased from Thermo-Fisher, France. Ultrapure water was purified by a PURELAB Flex system purchased from ELGA, UK. Arginylglycylaspartic acid grafted poly-l-lysine-graft-poly (ethylene glycol) (PLL-g-PEG/PEG-RGD) was purchased from SuSoS, Switzerland.