Pierce classic magnetic immunoprecipitation ip co ip kit

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Pierce Classic Magnetic Immunoprecipitation (IP)/Co-IP Kit is a laboratory tool designed for the isolation and purification of target proteins from complex biological samples using magnetic beads. The kit provides the necessary reagents and components to perform immunoprecipitation and co-immunoprecipitation experiments.

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Pierce Classic Magnetic IP/Co-IP Kit PierceTM Classic Magnetic Co-IP kit Pierce Co-Immunoprecipitation (Co-IP) Kit Classic Magnetic IP/Co-IP kit Pierce Classic Magnetic IP Kit Co-Immunoprecipitation (Co-IP) Kit Pierce Classic Immunoprecipitation Kit Pierce Classic IP Kit Pierce Co-Immunoprecipitation Kit Pierce Co-IP kit

2 protocols using pierce classic magnetic immunoprecipitation ip co ip kit

Magnetic IP-Western Blot Assay

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The IP assay was performed using Pierce Classic Magnetic immunoprecipitation (IP)/Co-IP Kit (Thermo Fisher, USA) according to the manufacturer’s instruction. Firstly, the different antibodies were incubated with protein A/G magnetic beads. Then the cell lysates from GSCs were collected and incubated with antibody coupled beads. The beads interacting proteins were washed and denatured and the proteins were examined using western blotting.

Gao Z., Xu J., Fan Y., Qi Y., Wang S., Zhao S., Guo X., Xue H., Deng L., Zhao R., Sun C., Zhang P, & Li G. (2022). PDIA3P1 promotes Temozolomide resistance in glioblastoma by inhibiting C/EBPβ degradation to facilitate proneural-to-mesenchymal transition. Journal of Experimental & Clinical Cancer Research : CR, 41, 223.

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Magnetic IP-based Protein Extraction from Glioma Cells

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The protein of glioma cells was obtained with a Pierce™ Classic Magnetic immunoprecipitation (IP)/Co‐IP Kit (Thermo Fisher). Then, western blotting was performed as indicated.

Wang H., Chen Z., Wang S., Gao X., Qian M., Qiu W., Zhang Z., Zhang S., Qi Y., Sun X., Xue H., Guo X., Zhao R, & Li G. (2020). TGFβ1‐induced beta‐site APP‐cleaving enzyme 2 upregulation promotes tumorigenesis through the NF‐κB signalling pathway in human gliomas. Molecular Oncology, 14(2), 407-425.

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