Cells were lysed with RIPA buffer (50 mM Tris-HCl pH 8.0, 150 mM NaCl, 0.1% SDS, 1% Nonidet P40, supplemented with Roche Complete protease inhibitor cocktail). Protein concentration was measured by using Comassie Plus protein assay reagent (Thermo Scientific), and equal amounts of proteins were separated by SDS-PAGE electrophoresis and transferred onto a nitrocellulose membrane by standard methods. Membranes were incubated with anti-GAPDH (Santa Cruz Biotechnology, Santa Cruz, CA), anti-ANXA8 (LifeSpan Biosciences, Seattle, WA) or anti-ANXA2 (BD Biosciences), washed, and incubated with an appropriate HRP-conjugated antibody (GE Healthcare, Piscataway, NJ) followed by ECL detection (GE Healthcare). Protein band intensity was quantified by using Image J (NIH). Statistical significance was calculated by using the Student’s t-test.
Anti anxa8
Manufactured by LifeSpan BioSciences
Sourced in Canada, United States
Anti-ANXA8 is an antibody product offered by LifeSpan BioSciences. It is designed to detect the presence of the ANXA8 protein in samples.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using anti anxa8
1
Quantitative Western Blot Analysis
2
Western Blot Quantification Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were lysed with RIPA buffer (50 mM Tris-HCl pH 8.0, 150 mM NaCl, 0.1% SDS, 1% Nonidet P40, supplemented with Roche Complete protease inhibitor cocktail). Protein concentration was measured by using Coomassie Plus protein assay reagent (Thermo Scientific, Waltham, Massachusetts, USA,), and equal amounts of proteins were separated by SDS-PAGE electrophoresis and transferred onto a nitrocellulose membrane by standard methods. Membranes were incubated with anti-β-tubulin (Santa Cruz Biotechnology, Santa Cruz, CA, USA) or anti-ANXA8 (LifeSpan Biosciences, Seattle, WA, USA) washed, and incubated with an appropriate HRP-conjugated antibody (GE Healthcare, Piscataway, NJ, USA) followed by ECL detection (GE Healthcare). Protein band intensity was quantified by using Image J (NIH).
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