Total RNA was extracted using RNeasy mini kit for mRNA and miRNeasy mini kit for miRNA (QIAGEN). 500ng (mRNA) or 50ng (miRNA) was reverse transcribed into cDNA using iScript cDNA Synthesis Kit (Bio-Rad) for mRNA or the TaqMan microRNA Reverse Transcription Kit (Applied Biosystems) for miRNA. For mRNA, cDNA PCR was performed using SYBR Green master mix (Applied Biosystems). For miRNA, cDNA PCR was done using TaqMan Universal Master Mix, no AmpErase UNG (Applied Biosystems) and primers (20X) from the TaqMan MicroRNA Assay. Reactions were run in quadruplicates. Data were normalized to housekeeping genes GAPDH or GUSB for mRNA and RNU48 for miRNA and represented as mean ± SD. For mir-34A methylation analysis, genomic DNA was extracted using DNeasy Blood & Tissue kit (QIAGEN). Bisulfite conversion was performed with EpiTect kit (QIAGEN) and CpG island 157 was amplified by PCR. The PCR products were gel-purified and subcloned into pGEM-T Easy vector (Promega) followed by transformation into competent cells for blue-white screening. 8–10 positive colonies were sequenced with T7 primers. All relevant primer sequences are listed in Supplementary Methods .
Mirneasy mini kit for mirna
Manufactured by Qiagen
Sourced in Germany
The MiRNeasy mini kit is a product from Qiagen that is used for the purification of microRNA (miRNA) from various sample types. The kit utilizes a silica-membrane-based technology to isolate and purify miRNA from a wide range of biological samples, including cells, tissues, and body fluids.
Automatically generated - may contain errors
Lab products found in correlation
No amperase ung, by Thermo Fisher Scientific (1 mentions)
Epitect kit, by Qiagen (1 mentions)
Taqman microrna reverse transcription kit, by Thermo Fisher Scientific (1 mentions)
Rneasy mini kit for mrna, by Qiagen (1 mentions)
Taqman universal master mix, by Thermo Fisher Scientific (1 mentions)
Sybr green master mix, by Thermo Fisher Scientific (1 mentions)
Dneasy blood tissue kit, by Qiagen (1 mentions)
Iscript cdna synthesis kit, by Bio-Rad (1 mentions)
Pgem t easy vector, by Promega (1 mentions)
Rna 6000 nano labchipvr reagent set, by Agilent Technologies (1 mentions)
Microarray scanner g2505b, by Agilent Technologies (1 mentions)
Gene expression hybridisation kit, by Agilent Technologies (1 mentions)
Rneasy mini kit, by Qiagen (1 mentions)
2 protocols using mirneasy mini kit for mirna
1
Quantification of mRNA and miRNA Expression
2
RNA Isolation and Microarray Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
RNA isolation was performed according to the manufacturer's instructions using the RNeasy Mini-Kit (Qiagen, Hilden, Germany) for mRNA and miRNeasy Mini-Kit for miRNA (Qiagen). The prepared RNA was preserved at −80 °C. Only samples with RNA integrity number >8.8 for miRNA and >8.7 for mRNA were accepted for further processing.
Array analyses were performed using RNA 6000 Nano LabChipVR reagent set (Agilent Technologies, Boeblingen, Germany), Low RNA Input Linear Amplification Kit PLUS, One-Color (Agilent Technologies), Gene Expression Hybridisation Kit (Agilent) and miRNA Labelling Reagent and Hybridisation Kit (Agilent Technologies).
The Microarray Scanner G2505B (Agilent Technologies) was used for washing and scanning of miRNA and mRNA arrays.
Array analyses were performed using RNA 6000 Nano LabChipVR reagent set (Agilent Technologies, Boeblingen, Germany), Low RNA Input Linear Amplification Kit PLUS, One-Color (Agilent Technologies), Gene Expression Hybridisation Kit (Agilent) and miRNA Labelling Reagent and Hybridisation Kit (Agilent Technologies).
The Microarray Scanner G2505B (Agilent Technologies) was used for washing and scanning of miRNA and mRNA arrays.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!