Cells were seeded at 24 well plates overnight and were then treated with different nanoparticles for 48 h. For gene expression, total RNA of cells was isolated and then PAR2 gene expression was detected by RT-PCR. For cell surface expression of PAR2, cells were incubated with the primary antibody against PAR2 in PBS for 1 h and were then incubated with Cy3-conjugated affinipure goat anti-rabbit IgG followed by fluorescence detection using flow cytometry analysis (Becton Dickinson). For PAR2 protein expression, lung tissues were lysed and detected by Western blotting technique after drug treatments.
Cy3 conjugated affinipure goat anti rabbit igg
Manufactured by BD
Cy3-conjugated affinipure goat anti-rabbit IgG is a secondary antibody that binds to rabbit immunoglobulin G (IgG). The Cy3 fluorescent dye is conjugated to the antibody, allowing for the detection and visualization of rabbit IgG in various laboratory applications.
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2 protocols using cy3 conjugated affinipure goat anti rabbit igg
1
Characterization of PAR2 Expression
2
Characterization of PAR2 Expression
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Cells were seeded at 24 well plates overnight and were then treated with different nanoparticles for 48 h. For gene expression, total RNA of cells was isolated and then PAR2 gene expression was detected by RT-PCR. For cell surface expression of PAR2, cells were incubated with the primary antibody against PAR2 in PBS for 1 h and were then incubated with Cy3-conjugated affinipure goat anti-rabbit IgG followed by fluorescence detection using flow cytometry analysis (Becton Dickinson). For PAR2 protein expression, lung tissues were lysed and detected by Western blotting technique after drug treatments.
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