Cd80 alexa647

Manufactured by BioLegend

CD80-Alexa647 is a fluorescently labeled antibody that binds to the CD80 surface protein. CD80 is a co-stimulatory molecule expressed on activated antigen-presenting cells. The Alexa Fluor 647 fluorophore is conjugated to the CD80 antibody, enabling detection and analysis by flow cytometry or other fluorescence-based techniques.

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Lab products found in correlation

Cd69 percpcy5, by BioLegend (1 mentions) Cd3 alexa 488, by BioLegend (1 mentions) Ready set go elisa kit, by Thermo Fisher Scientific (1 mentions) Cd86 percp cy5, by BioLegend (1 mentions) Anti cd11c alexa488, by BioLegend (1 mentions) Cd19 alexa647, by BioLegend (1 mentions) Facscalibur flow cytometry, by BD (1 mentions) Ccr3 apc, by BioLegend (1 mentions) Cd3 percp, by Thermo Fisher Scientific (1 mentions) Cd15 pe cy7, by BioLegend (1 mentions) Cd3 apc cy7, by BioLegend (1 mentions) Cd8 percp, by Thermo Fisher Scientific (1 mentions) Fixable viability dye efluor 780, by Thermo Fisher Scientific (1 mentions) Facsdiva, by BD (1 mentions) Annexin 5 fitc, by BD (1 mentions) Cd45 fitc, by BD (1 mentions)

2 protocols using cd80 alexa647

Immunomodulatory Effects of GD5 and DOX

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One week after the tumor injection, the tumor-bearing mice were treated intraperitoneally with 2.5 mg/kg GD5 or 3.5 mg/kg DOX. After 2 and 4 h, the spleens were collected, and the splenocytes were prepared according to the manufacturer's instructions (BioLegend). Approximately 1 × 10⁶ cells were stained with surface antibodies and analyzed by FACSCalibur flow cytometry (BD Biosciences). The antibodies including anti-CD11c-Alexa488, -CD86-PerCP/Cy5.5, -CD80-Alexa647, -CD3-Alexa488, -CD19-Alexa647 and -CD69-PerCP/Cy5.5 were purchased from BioLegend.
Sera samples were collected at 2, 4 or 24 h from the same administrated mice and stored at -20°C. ELISA was performed using a Ready-SET-Go!^® ELISA kit (eBioscience).

Gao D., Li W., Wang W., Cai Y., Wang Y., Luo X, & Wei C.C. (2017). Synergy of purine-scaffold TLR7 agonist with doxorubicin on systemic inhibition of lymphoma in mouse model. Journal of Cancer, 8(16), 3183-3189.

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Multiparameter Flow Cytometry Analysis

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Analyses were performed with a FACS Canto II using FACS Diva (BD Biosciences, San Jose, CA, USA) and FlowJo software (TreeStar, Inc., Ashland, OR, USA). The following anti-human antibodies were used: HLA-DR-BV421™, HLA-DR-APC (clone L243), CD80-Alexa647, CD3-APC-Cy7, CD16-PE (clone 3G8), CD15-PE-Cy7, CD15-PerCp-Cy5, CCR3-APC and PerCP-labelled CD14, CD19, CD56, CD123, and CCR3 (all from Biolegend, San Diego, CA), CD4-PE and CD86-PE, CD45-FITC (all from BD Biosciences) and CD3-PerCP and CD8-PerCP (both from eBioscience Inc., San Diego, CA). Viability was determined by using the Fixable Viability Dye eFluor® 780 (eBioscience Inc.). Annexin V-FITC (BD Pharmingen, San Jose, CA, USA) was used to determine cells undergoing apoptosis. For internalization experiments, cells (1x10⁶/ml) were incubated with and without pHrodo-labelled Bet v 1 (1.5 μg/ml) in the presence of GM-CSF/IFN-γ/IL-3.

Polak D., Hafner C., Briza P., Kitzmüller C., Elbe-Bürger A., Samadi N., Gschwandtner M., Pfützner W., Zlabinger G.J., Jahn-Schmid B, & Bohle B. (2018). A novel role for neutrophils in IgE-mediated allergy: evidence for antigen-presentation in late-phase reactions. The Journal of allergy and clinical immunology, 143(3), 1143-1152.e4.

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