Qiasymphony and the dsp virus pathogen mini kit

Manufactured by Qiagen

The QIAsymphony is an automated sample processing instrument designed for efficient and reliable nucleic acid purification. The DSP Virus/Pathogen Mini Kit is a reagent kit compatible with the QIAsymphony platform, used for the isolation and purification of viral nucleic acids from various sample types.

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Lab products found in correlation

Universal viral transport media system, by BD (2 mentions) Rotor gene q thermocycler, by Qiagen (1 mentions)

3 protocols using qiasymphony and the dsp virus pathogen mini kit

Nasopharyngeal Swab SARS-CoV-2 Extraction

Cited 1 time

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Patient specimens were processed as described in Butler et al. (2021) (link). Briefly, nasopharyngeal swabs were collected using the BD Universal Viral Transport Media system (Becton, Dickinson and Company, Franklin Lakes, NJ) from symptomatic patients. Total Nucleic Acid (TNA) was extracted from using automated nucleic acid extraction on the QIAsymphony and the DSP Virus/Pathogen Mini Kit (QIAGEN).

McDonald J.T., Enguita F.J., Taylor D., Griffin R.J., Priebe W., Emmett M.R., Sajadi M.M., Harris A.D., Clement J., Dybas J.M., Aykin-Burns N., Guarnieri J.W., Singh L.N., Grabham P., Baylin S.B., Yousey A., Pearson A.N., Corry P.M., Saravia-Butler A., Aunins T.R., Sharma S., Nagpal P., Meydan C., Foox J., Mozsary C., Cerqueira B., Zaksas V., Singh U., Wurtele E.S., Costes S.V., Davanzo G.G., Galeano D., Paccanaro A., Meinig S.L., Hagan R.S., Bowman N.M., Wolfgang M.C., Altinok S., Sapoval N., Treangen T.J., Moraes-Vieira P.M., Vanderburg C., Wallace D.C., Schisler J.C., Mason C.E., Chatterjee A., Meller R, & Beheshti A. (2021). Role of miR-2392 in driving SARS-CoV-2 infection. Cell Reports, 37(3), 109839.

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SARS-CoV-2 Nasopharyngeal Specimen Processing

Cited 1 time

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Saravia-Butler A.M., Schisler J.C., Taylor D., Beheshti A., Butler D., Meydan C., Foox J., Hernandez K., Mozsary C., Mason C.E, & Meller R. (2022). Host transcriptional responses in nasal swabs identify potential SARS-CoV-2 infection in PCR negative patients. iScience, 25(11), 105310.

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SARS-CoV-2 Viral RNA Extraction and Detection

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Total viral RNA was extracted from deactivated samples using automated nucleic acid extraction on the QIAsymphony and the DSP Virus/Pathogen Mini kit (Qiagen). One-step reverse transcription to complementary DNA and real-time PCR amplification of viral targets, E (envelope) and S (spike) genes and internal control, was performed using the Rotor-Gene Q thermocycler (Qiagen).

Ramlall V., Thangaraj P.M., Meydan C., Foox J., Butler D., Kim J., May B., De Freitas J.K., Glicksberg B.S., Mason C.E., Tatonetti N.P, & Shapira S.D. (2020). Immune complement and coagulation dysfunction in adverse outcomes of SARS-CoV-2 infection. Nature medicine, 26(10), 1609-1615.

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