Rna 6000 nano reagents kit

Manufactured by Agilent Technologies

Sourced in Canada, Lithuania, Germany

The RNA 6000 Nano Reagents Kit is a laboratory equipment product designed for the analysis of RNA samples. The kit contains the necessary reagents and components required to perform RNA integrity assessment and quantitation using the Agilent Bioanalyzer system.

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Lab products found in correlation

Rneasy mini kit, by Qiagen (2 mentions) Amino allyl messageamp 2 arna amplification kit, by Thermo Fisher Scientific (1 mentions) Messageamp 2 arna amplification kit, by Thermo Fisher Scientific (1 mentions) Pbr322, by Thermo Fisher Scientific (1 mentions) Cy3 mono reactive dye, by GE Healthcare (1 mentions) Iscript cdna synthesis kit, by Bio-Rad (1 mentions) Agilent 2100 bioanalyzer instrument, by Agilent Technologies (1 mentions) High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (1 mentions) Qx200 droplet digital pcr system, by Bio-Rad (1 mentions) Nanodrop 2000 spectrophotometer, by Thermo Fisher Scientific (1 mentions) Agilent 2100 bioanalyser, by Agilent Technologies (1 mentions) Mouse genechip microarrays mogene 1, by Thermo Fisher Scientific (1 mentions) Nanophotometer p330, by Implen (1 mentions) Rq1 rnase free dnase, by Promega (1 mentions) Agilent 2100 bioanalyzer, by Agilent Technologies (1 mentions) 2100 bioanalyzer, by Agilent Technologies (1 mentions) Trizol extraction reagent, by Omega Bio-Tek (1 mentions) Chloroform, by Carlo Erba (1 mentions)

Spelling variants of this product

RNA 6000 Nano (61 citations) RNA Nano kit (41 citations) RNA 6000 Nano Reagents (15 citations) RNA Nano reagents (5 citations) Nano 6000 kit (2 citations)

5 protocols using rna 6000 nano reagents kit

RNA Amplification and Microarray Processing

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Sample amplification, labeling, and microarray processing were performed by the Covance Genomics Laboratory (Seattle, WA). Briefly, samples were amplified using a custom two-cycle RT-IVT amplification protocol. For each sample, 5ng of total RNA was mixed with 250ng of pBR322 (Life Technologies) to act as a carrier. The MessageAmp II aRNA Amplification Kit was utilized for the first round of amplification and the Amino Allyl MessageAmp II aRNA Amplification Kit for the second round of amplification (Life Technologies). Following amplification, 5µg of cRNA was labeled with Cy3 mono-Reactive Dye (GE Healthcare). Each labeled aRNA was resolved using a Bioanalyzer with RNA 6000 Nano kit reagents (Agilent Technologies) before hybridization. Samples were evaluated for yield and size distribution, then normalized to 600ng input, fragmented, and hybridized to Agilent Human 8×60K Arrays. Gene expression data quality was assessed using standard Agilent quality control metrics. To control for batch effects, common RNA pool control samples were amplified and hybridized in each batch. A total of 1,225 samples passed sample quality control (QC), including 1,202 experimental samples and 23 control samples. The data discussed in this publication are accessible through the Allen Brain Atlas data portal (http://www.brain-map.org) or directly at http://www.brainspan.org.

Miller J.A., Ding S.L., Sunkin S.M., Smith K.A., Ng L., Szafer A., Ebbert A., Riley Z.L., Aiona K., Arnold J.M., Bennet C., Bertagnolli D., Brouner K., Butler S., Caldejon S., Carey A., Cuhaciyan C., Dalley R.A., Dee N., Dolbeare T.A., Facer B.A., Feng D., Fliss T.P., Gee G., Goldy J., Gourley L., Gregor B.W., Gu G., Howard R.E., Jochim J.M., Kuan C.L., Lau C., Lee C.K., Lee F., Lemon T.A., Lesnar P., McMurray B., Mastan N., Mosqueda N.F., Naluai-Cecchini T., Ngo N.K., Nyhus J., Oldre A., Olson E., Parente J., Parker P.D., Parry S.E., Player A.S., Pletikos M., Reding M., Royall J.J., Roll K., Sandman D., Sarreal M., Shapouri S., Shapovalova N.V., Shen E.H., Sjoquist N., Slaughterbeck C.R., Smith M., Sodt A.J., Williams D., Zöllei L., Fischl B., Gerstein M.B., Geschwind D.H., Glass I.A., Hawrylycz M.J., Hevner R.F., Huang H., Jones A.R., Knowles J.A., Levitt P., Phillips J.W., Sestan N., Wohnoutka P., Dang C., Bernard A., Hohmann J.G, & Lein E.S. (2014). Transcriptional Landscape of the Prenatal Human Brain. Nature, 508(7495), 199-206.

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Droplet Digital PCR Analysis of Mouse Hypothalami

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Five wildtype C57/Bl6 mice were euthanized with 2% CO₂, and their brains and livers were rapidly extracted. The hypothalamus from each animal was sub sectioned and harvested as a ~17 mm³ block. Hypothalami were blocked coronally from the preoptic area (+0.26 mm from bregma) to the midbrain (−3.52 mm from bregma), sagitally from optic tract-to-optic tract (+/− 1.5 mm from midline), and horizontally through the ventral thalamus (1.5 mm from the ventral brain surface). RNA was extracted from tissues from 5 animals using an RNeasy mini kit (Qiagen, Hilden, Germany), and quality control for all samples was performed using an Agilent 2100 Bioanalyzer Instrument (Agilent, Santa Clara, CA) and RNA 6000 nano kit reagents (Agilent). All samples had an RNA integrity number exceeding 8.0 (on a 1–10 scale based on 28S and 18S ribosome electropherogram data) and were included for ddPCR assays. For these assays, 10–100 ng of cDNA was made using a high capacity cDNA reverse transcription kit (Applied Biosystems, Foster City, CA) or the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA). All samples were assayed in triplicate. ddPCR was performed on a QX200 droplet digital PCR system (Bio-Rad), and data output for positive ddPCR read These assays utilized the same probe sets from Applied Biosystems outlined in Table 3.

Hultman K., Scarlett J.M., Baquero A.F., Cornea A., Zhang Y., Salinas C.B., Brown J., Morton G.J., Whalen E.J., Grove K.L., Koegler F.H., Schwartz M.W, & Mercer A.J. (2019). The central fibroblast growth factor receptor/beta klotho system: comprehensive mapping in mus musculus and comparisons to non-human primate and human samples using an automated in situ hybridization platform. The Journal of comparative neurology, 527(12), 2069-2085.

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Transcriptomic Analysis of CD2-MYC/Runx2 Mice

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RNA was isolated and purified from the thymuses of 10 day old wild type and CD2-MYC/Runx2 double transgenic mice using an RNeasy Mini Kit as per the manufacturer's instructions (Qiagen, UK) with mechanical lysis using a pellet pestle in a microfuge tube (Sigma). RNA purity was assessed using a Nanodrop 2000 Spectrophotometer (Thermo Scientific), and integrity verified using the Agilent 2100 Bioanalyser with RNA 6000 Nano Reagents kit (Agilent Biotechnologies) as per the manufacturer's protocol. Whole genome expression profiling was performed using Affymetrix mouse GeneChip microarrays (MoGene-1) in triplicate as per the manufacturer's protocol (Affymetrix, UK). Data analysis was carried out using the Partek Genomic Suite (Partek Inc., St. Louis, MO, USA). Briefly, after Robust Multichip Average normalisation [76] (link) with GC content pre-background adjustment, the differentially expression analysis was performed using ANOVA. Multiple testing correction was done using the ‘q value’ cut-off [77] with gene changes of p<0.05 considered significant. Graphical representations of data were prepared using CLC Genomics Workbench 4.

Huser C.A., Gilroy K.L., de Ridder J., Kilbey A., Borland G., Mackay N., Jenkins A., Bell M., Herzyk P., van der Weyden L., Adams D.J., Rust A.G., Cameron E, & Neil J.C. (2014). Insertional Mutagenesis and Deep Profiling Reveals Gene Hierarchies and a Myc/p53-Dependent Bottleneck in Lymphomagenesis. PLoS Genetics, 10(2), e1004167.

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Pepper Flower and Fruit RNA Extraction

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Samples were collected from two-year-old mature pepper plants (Piper nigrum L.) of SA, S1, and KC varieties at a pepper farm in Kampong Karu, Borneo Highland Sarawak, Malaysia. Fresh flowers (1 day after anthesis, DAA) and fruit spikes (14 DAA) (Supplementary ) were collected from two biological replicates (two plants) of each pepper variety and snap-frozen in liquid nitrogen before storage at -80°C until use. Total RNA was extracted from 2 g of frozen plant tissue using a modified CTAB method [18 (no link found, link)]. The RNA was purified and treated with RQ1 RNase-free DNase (Promega, USA) to remove DNA contamination. The RNA purity and concentration were measured at 260/230 nm and 260/280 nm using a spectrophotometer (NanoPhotometer P330, IMPLEN, Germany) while the RNA integrity was measured on an Agilent 2100 Bioanalyzer with RNA 6000 Nano Reagents Kit (Agilent Technologies, 5067-1511, Lithuania). The RNA Integrity Number (RIN) was calculated using an algorithm adapted for plant RNA profiles. All RIN values were between 7.4 and 8.5.

Khew C.Y., Harikrishna J.A., Wee W.Y., Lau E.T, & Hwang S.S. (2020). Transcriptional Sequencing and Gene Expression Analysis of Various Genes in Fruit Development of Three Different Black Pepper (Piper nigrum L.) Varieties. International Journal of Genomics, 2020, 1540915.

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CNT-Induced RNA Extraction Protocol

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After NR8383 cells treatment for 4 hours with the IC₅₀ and IC_50/4 of each CNT, cells were lysed by adding 1 mL of Trizol Extraction Reagent (OMEGA Bio‐Tek, Guang zhou, China), followed by the addition of 200 μL of chloroform (Carlo Erba reagents, Normandie, France). Samples were centrifuged at 800 g for 15 minutes and 500 μL of isopropanol (Carlo Erba reagents) was added to 350 μL of supernatant. The precipitates were subjected to two washing steps using ethanol 80% and incubated for 10 minutes at 60°C to remove ethanol, followed by dissolution in 35 μL RNase‐free water.
RNA purity was assessed using a BioSpec‐nano spectrophotometer (Shimadzu, Kyoto, Japan). In addition, the integrity of RNA was checked by RNA 6000 Nano Reagents Kit using Bioanalyzer™ 2100 (Agilent Technologies, Waldbron, Germany).

Nahle S., Safar R., Grandemange S., Foliguet B., Lovera‐Leroux M., Doumandji Z., Le Faou A., Joubert O., Rihn B, & Ferrari L. (2019). Single wall and multiwall carbon nanotubes induce different toxicological responses in rat alveolar macrophages. Journal of Applied Toxicology, 39(5), 764-772.

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