Proinflammatory cytokines known to be involved in Inflammaging [4 (link)–8 ] (TNFα, IL-1β and IL-6, IL-8, IL-12 and sTNF RI) and to be important for the pathogenesis of bronchial asthma were selected for testing. The study also included IL-10, a cytokine with immunoregulatory and antagonistic properties. The serum levels of TNF-α, IL-1β and IL-6 were determined with the use of the commercial Thermo Fisher Scientific Ultrasensitive TNF alpha Human ELISA Kit, High Sensitivity IL-1 beta Human ELISA Kit and High Sensitivity IL-6 Human ELISA Kit. The serum level of soluble TNF RI was determined with the use of the commercial R&D Systems Human TNF RI/TNFRSF1A Quantikine® ELISA Kit. IL-12p70, IL-10 and IL-8 levels in the serum were measured with the BD™ Cytometric Bead Array Human Inflammatory Cytokine Kit. The samples were run on a BD™ LSR Fortessa flow cytometer and the results were analyzed with the BD™ FCAP Array software.
IL-10 was detectable only in 48/118 (40.7%), IL-12p70 in 20/118 (16.9%) and IL-1β in 10/118 (8.5%) of the study participants, and IL-10, IL-12p70 and IL-1β were not included in the analysis.
IL-10 was detectable only in 48/118 (40.7%), IL-12p70 in 20/118 (16.9%) and IL-1β in 10/118 (8.5%) of the study participants, and IL-10, IL-12p70 and IL-1β were not included in the analysis.