Brightfield and fluorescence microscopy was performed using an Olympus BX50 fluorescence microscope (mercury source) equipped with a BX-FLA reflected light attachment and vertical illuminator. Lumogallion fluorescence was acquired by use of a U-MNIB3 fluorescence filter cube (bandpass λex: 470–495 nm, dichromatic mirror: 505 nm, longpass λem: 510 nm) and ThS fluorescence by use of a U-MWBV2 cube (bandpass λex: 440–440 nm, dichromatic mirror: 455 nm, longpass λem: 475 nm, Olympus, UK). Ultraviolet (UV) induced protein autofluorescence was collected using a U-MWU2 cube (bandpass λex: 330–385 nm, dichromatic mirror: 400 nm, longpass λem: 420 nm, Olympus, UK). Images were captured using an Olympus DP74 (CMOS processor) colour camera and the OLYMPUS cellSens (Standard 3.1) software suite. Optimal exposure times for collecting fluorescence micrographs were automatically set by the superfluorescence (SFL) mode of the Olympus DP74 camera. Optimised exposure settings were subsequently fixed for each corresponding autofluorescence (U-MNIB3) micrograph. Merging of fluorescence and brightfield channels was achieved using Photoshop (Adobe Systems Inc., USA).
U mwbv2 cube
Manufactured by Olympus
Sourced in United Kingdom
The U-MWBV2 cube is a versatile laboratory equipment designed for various applications. It features an advanced optical system that enables precise microscopic observations. The cube's compact and durable construction makes it suitable for use in diverse research and analytical settings.
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Lab products found in correlation
Bx50 fluorescence microscope, by Olympus (2 mentions)
Cellsens, by Olympus (1 mentions)
U mwu2 cube, by Olympus (1 mentions)
U mnib3 fluorescence filter cube, by Olympus (1 mentions)
U ant transmitted light analyzer, by Olympus (1 mentions)
Celld software suite, by Olympus (1 mentions)
Color view 3, by Olympus (1 mentions)
2 protocols using u mwbv2 cube
1
Microscopy Techniques for Fluorescence Imaging
2
Multimodal Microscopy for Biomolecular Imaging
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Fluorescence, brightfield, and polarized light microscopy were performed via use of an Olympus BX50 fluorescence microscope (mercury source), equipped with a BX-FLA reflected light attachment. Lumogallion fluorescence was collected through an Olympus U-MNIB3 fluorescence filter cube (excitation filter: 470-495 nm, dichromatic mirror: 505 nm, longpass emission filter: 510 nm) and ThS fluorescence collected by use of an Olympus U-MWBV2 cube (excitation filter: 400-440 nm, dichromatic mirror: 455 nm, longpass emission filter: 475 nm). Polarized light illumination was achieved by use of a U-POT drop-in polarizer and a U-ANT transmitted light analyzer (both from Olympus, UK). Images were acquired by use of a ColorView III charged coupled device (CCD) camera and the CellD software suite (Olympus, SiS Imaging Solutions, GmbH). Fluorescence channels were merged using Photoshop (Adobe Systems Inc., USA).
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