Anti ubiquitin antibody z0458

Manufactured by Agilent Technologies

Sourced in Canada

The Anti-ubiquitin antibody (Z0458) is a laboratory equipment product manufactured by Agilent Technologies. It is designed to detect and analyze ubiquitin, a small regulatory protein found in eukaryotic cells.

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Lab products found in correlation

Sc 32282, by Santa Cruz Biotechnology (1 mentions) Anti actin a2066, by Merck Group (1 mentions) Anti flag hrp a8592, by Merck Group (1 mentions)

Spelling variants of this product

Ubiquitin (36 citations) Anti-ubiquitin (35 citations) Z0458 (16 citations) Anti-ubiquitin antibody (9 citations) Anti-Ubiquitin Z0458 (2 citations) Ubiquitin antibody (2 citations) Anti-TM (1 citations)

2 protocols using anti ubiquitin antibody z0458

Antibody Characterization and Validation

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Antigen affinity-purified sheep anti-SUMO-1 antibody was a kind gift from Professor Ron Hay (Dundee) (IB: 1:2,000). Anti-Parkin mouse monoclonal was obtained from Santa Cruz (sc-32282) (IB: 1:5,000; IF: 1:1,000); anti-FLAG HRP (A8592) (IB: 1:10,000) and anti-actin (A2066) (IB: 1:5,000) antibodies were obtained from Sigma; anti-CISD1 (16006-1-AP) (IB: 1:1,000) and anti-TOMM70A (14528-1-AP) (IB: 1:1,000) antibodies were obtained from Proteintech Europe; and anti-ubiquitin antibody (Z0458) (IB: 1:1,000) was purchased from Dako. Epitomics raised anti-Parkin phospho-serine 65 rabbit monoclonal antibody in collaboration with the Michael J Fox Foundation for Research (IB: 1:1,000; IF: 1:500).

Kazlauskaite A., Martínez-Torres R.J., Wilkie S., Kumar A., Peltier J., Gonzalez A., Johnson C., Zhang J., Hope A.G., Peggie M., Trost M., van Aalten D.M., Alessi D.R., Prescott A.R., Knebel A., Walden H, & Muqit M.M. (2015). Binding to serine 65-phosphorylated ubiquitin primes Parkin for optimal PINK1-dependent phosphorylation and activation. EMBO Reports, 16(8), 939-954.

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Antibody Panel for Ubiquitin Pathway Analysis

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Anti-ubiquitin (P4D1, sc-8017), anti-Cbl (C-15, sc-170), anti-Cbl-b (H-121, sc-1704), anti-Cbl-b (H454, sc-1705), anti-Cbl-b (G1, sc-8006), anti-Ube2d (C-6, sc-166278), anti-GST (sc-138), anti-Ube2e2 (sc-130052), anti-HSC70 (sc-7298) antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). The anti-Ube2e1 (ab36980) antibody was from Abcam (Cambridge, MA). The anti-Ube2e3 (MABS17), anti-Syk (05–434), anti-Src (04–889), anti-ubiquitin K63-specific (05–1308), and K48-specific (05–1307) antibodies were obtained from EMD Millipore (Burlington, MA). The anti-Ube2w (HPA045161), anti-actin (A5316), and anti-FLAG (A8592) antibodies were obtained from MilliporeSigma (St. Louis, MO). The anti-EGFR antibody (199.12, MA5-13319) was purchased from ThermoFisher Scientific (Waltham, MA) and used for immunoprecipitation. The anti-EGFR (2232 and 4276) antibodies were obtained from Cell Signaling Technology (Danvers, MA) and used for immunoblotting. The anti-ubiquitin antibody Z0458 was obtained from DAKO (Carpinteria, CA).

Liyasova M.S., Ma K., Voeller D., Ryan P.E., Chen J., Klevit R.E, & Lipkowitz S. (2019). Cbl interacts with multiple E2s in vitro and in cells. PLoS ONE, 14(5), e0216967.

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