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3 protocols using trans 3 octen 2 one

1

Analysis of Volatile Organic Compounds

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Chemical standards of acetone, butane-2,3-dione, butan-2-one, thiophene, dimethyl disulfide, hexanal, heptan-4-one, heptan-2-one, 2-pentylfuran, dimethyl trisulfide, 6-methylhept-5-en-2-one, benzaldehyde, octanal, d-limonene, trans-3-octen-2-one, linalool, 4-methylphenol, 1,3-di-tert-butylbenzene, internal standard (4-methylpentan-2-ol), and sodium chloride (NaCl, ≥99.5%) were supplied by Sigma-Aldrich (Saint Louis, MO, USA). MilliQ water (Millipore, Bedford, MO, USA) was used for the preparation of standards. Hydrochloric acid (HCl, 37%) was purchased from Chempur (Piekary Śląskie, Poland). The 75-μm carboxen/polydimethylsiloxane (CAR/PDMS) (stable flex) solid-phase microextraction (SPME) fibers were purchased from Supelco (Bellefonte, PA, USA).
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2

Volatile Compound Identification Standards

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Standards were purchased for volatile compound identification. 2-Pentylfuran and trans-3-octen-2-one were provided by Sigma Aldrich (St. Louis, MO, USA). Other compounds, including hexanal, benzaldehyde, octanal, nonanal, heptanal, 3-methylbutanal, propanol, pentanol, hexanol, ethanol, 1-octen-3-ol, 3-methyl-1-butanol, 2-methylpropanol, phenethyl alcohol, acetoin, 6-methyl-5-hepten-2-one, 2,3-butanedione, acetic acid, octanoic acid, pentanoic acid, hexanoic acid, γ-undecalactone, ethyl acetate, hexyl acetate, were provided by Macklin (Shanghai, China).
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3

Volatile Compounds Analysis via SPME

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Standards of all analyzed compounds: 2,3-butanedione, 2-butanone, thiophene, dimethyl disulfide, hexanal, 4-heptanone, 2-heptanone, 2-pentylfuran, dimethyl trisulfide, 6-methyl-5-hepten-2-one, benzaldehyde, octanal, trans-3-octen-2-one, p-cresol and 1,3-di-tert-butylbenzene, internal standard: 4-methyl-2-phenol and saturated alkanes (C7–C40) were supplied by Sigma-Aldrich (Saint Louis, USA). Retention indexes for selected compounds were calculated according to Zellner and co-authors.23 (link) MilliQ water (Millipore, Bedford, USA) was used for the preparation of standards. Sodium chloride (NaCl, ≥99.5%) was purchased from Sigma-Aldrich (Saint Louis, USA), sodium hydroxide (NaOH, 99%) from POCH (Gliwice, Poland) and hydrochloric acid (HCl, 37%) was purchased from Chempur (Piekary Śląskie, Poland). The SPME fibers used in the experiments were: 50/30 μm divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) (stable flex), 65 μm polydimethylsiloxane/divinylbenzene (PDMS/DVB) (stable flex), 7 μm polydimethylsiloxane (PDMS7), 100 μm polydimethylsiloxane (PDMS100), 75 μm carboxen/polydimethylsiloxane (CAR/PDMS) (stable flex), 85 μm polyacrylate (PA) (stable flex) and 60 μm carbowax-polyethylene glycol (PEG), purchased from Supelco (Bellefonte, PA, USA).
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