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Great escape seap chemiluminescence assay kit

Manufactured by Takara Bio
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The Great Escape SEAP Chemiluminescence Assay kit is a laboratory tool designed to quantify the activity of secreted embryonic alkaline phosphatase (SEAP) in cell culture supernatants. The kit utilizes a chemiluminescent substrate to detect and measure SEAP levels, providing a sensitive and versatile method for monitoring gene expression and cellular activity.

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Lab products found in correlation

Glomax 20 20 luminometer, by Promega (1 mentions) Lipofectamine ltx, by Thermo Fisher Scientific (1 mentions) Pnf κb seap, by Takara Bio (1 mentions) Cell culture media, by Corning (1 mentions) Matchmaker mammalian two hybrid assay kit, by Takara Bio (1 mentions) Enhanced chemiluminescence reagent, by GE Healthcare (1 mentions) Fetal bovine serum (fbs), by Corning (1 mentions) Annexin 5 fitc apoptosis detection kit, by Merck Group (1 mentions)

Spelling variants of this product

Great EscAPe SEAP Chemiluminescence Kit (13 citations) SEAP Great Escape chemiluminescence assay kit 2.0 (2 citations)

2 protocols using great escape seap chemiluminescence assay kit

1

Monitoring NF-κB and AP-1 Activation

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To monitor the activation of NF-κB and AP-1 signal transduction pathways, the plasmids (pNFκB-SEAP or pAP1-SEAP) were purchased from Clontech and were transiently transfected into MARC-145, MARC-2a, or MARC-N cells by the use of Lipofectamine LTX (Invitrogen). Briefly, 4 μg of each plasmid DNA, 12 μL Lipofectamine LTX, and 4 μL Plus reagent were used to transfect cells, seeded in a well of 6-well plate. Since these transfected plasmids contain the secreted alkaline phosphatase (SEAP) gene as a reporter, culture supernatants were collected 72 h after transfection, and SEAP activity was detected in by the Great Escape SEAP Chemiluminescence Assay kit (Clontech) using a GloMax 20/20 Luminometer (Promega). The chemiluminescence emitted by a SEAP-activated substrate (CSPD) was measured in relative luminescence units.
Pujhari S., Baig T.T, & Zakhartchouk A.N. (2014). Potential Role of Porcine Reproductive and Respiratory Syndrome Virus Structural Protein GP2 in Apoptosis Inhibition. BioMed Research International, 2014, 160505.
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2

Molecular Biology Assay Reagents

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Antibodies against HA tag (H6908) and actin (A5316) and the Annexin V-FITC apoptosis detection kit were obtained from MilliporeSigma. The antibody against survivin (2808) and the Metafectene Pro transfection reagent were purchased from Cell Signaling Technology and Bion-tex, respectively. The enhanced chemiluminescence reagent was from GE Healthcare. The matchmaker mammalian two hybrid assay kit and the Great Escape SEAP Chemiluminescence assay kit were purchased from Clontech. Cell culture media and fetal bovine serum were from Corning and Applied Biosystems-Life Technologies, respectively. All other chemicals were purchased from Sigma or Fisher Scientific.
Peery R., Kyei-Baffour K., Dong Z., Liu J., de Andrade Horn P., Dai M., Liu J.Y, & Zhang J.T. (2020). Synthesis and Identification of a Novel Lead Targeting Survivin Dimerization for Proteasome-Dependent Degradation. Journal of medicinal chemistry, 63(13), 7243-7251.
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