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Ubc gfp 004353

Manufactured by Jackson ImmunoResearch

UBC-GFP (004353) is a recombinant green fluorescent protein (GFP) fusion protein derived from the ubiquitin-conjugating enzyme Ubc9. It is designed for use as a control or reference protein in various applications, such as Western blotting, immunoassays, or other protein analysis techniques.

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2 protocols using ubc gfp 004353

1

Murine Models for B Cell Research

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B6 (C57BL/6) mice were purchased from Charles River Laboratory. B6-CD45.1 (Ptprca Pepcb/BoyJ) were purchased from the Jackson Laboratory. BCR transgenic HyHEL10 [19 (link)] and MD4 mice [20 (link)] were generously provided by Jason Cyster. HyHEL10 mice were crossed with UBC-GFP (004353) (Jackson Laboratory) and with B6-CD45.1 mice and maintained on the B6 background. MD4 mice were crossed with B6-CD45.1 and maintained on the B6 background. Recipient mice were 6–10 weeks of age. All mice were maintained in a specific pathogen free environment and protocols were approved by the Institutional Animal Care and Use Committee of the University of Michigan.
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2

Mouse Genetic Models for Research

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All animal experiments were approved by the Institutional Animal Care and Use Committees of the Emory University and Yonsei University College of Medicine, and were performed in accordance with federal guidelines. C57BL/6J wild-type (#000664) or transgenic mice carrying a GFP reporter driven by human ubiquitin C (UBC-GFP, #004353) promoters or an mCherry reporter driven by the MYH6 promoter (MYH6-mCherry, #021577) were purchased from Jackson Laboratory. Nkx2–5-GFP transgenic mice were kindly given by Dr. Richard P. Harvey (Victor Chang Cardiac Research Institute)56 (link).
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