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Itk ptyr512

Manufactured by Thermo Fisher Scientific
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ITK pTyr512 is a laboratory product manufactured by Thermo Fisher Scientific. It is a phosphorylated peptide, specifically phosphorylated on tyrosine residue 512. This product is intended for use in biochemical and cell-based assays.

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Lab products found in correlation

Supersignal west femto chemiluminescent substrate, by Thermo Fisher Scientific (2 mentions) Amersham ecl prime reagent, by GE Healthcare (2 mentions) Pan py, by Cell Signaling Technology (2 mentions) Cbl py674, by Abcam (2 mentions) Lat py255, by Thermo Fisher Scientific (2 mentions) Gapdh, by Cell Signaling Technology (2 mentions) Fluor s multiimager, by Bio-Rad (2 mentions) Amersham ecl prime, by Cytiva (2 mentions)

2 protocols using itk ptyr512

1

Western Blot Analysis of Signaling Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blot analysis used the following rabbit anti-human antibodies: PRL3 (GTX 100600, VWR # 89307–822, Radnor, Pennsylvania), VAV1 (Cell Signaling Technology #2502, Danvers, Massachusetts), Pan pY (Cell Signaling Technology #8954), and GAPDH (Cell Signaling Technology #2118), CBL (Cell Signaling Technology # 2747), CBL pY674 (Abcam # ab76536, Cambridge, United Kingdom), ITK (Cell Signaling Technology # 77215S), ITK pTyr512 (Life Technologies #PA564523), LAT pY255 ( Thermo Fisher Scientific # PIPA564800), LAT (Cell Signaling Technology # 9166S). Antibody binding was visualized with anti-rabbit HRP and developed by either Amersham ECL Prime reagent (GE Healthcare, Buckinghamshire, United Kingdom) or SuperSignal West Femto Chemiluminescent Substrate (Thermo Fisher Scientific) using a Fluor-S Multi-Imager (Bio-Rad, Hercules, California).
Garcia E.G., Veloso A., Oliveira M.L., Allen J.R., Loontiens S., Brunson D., Do D., Yan C., Morris R., Iyer S., Garcia S.P., Iftimia N., a L.W., Matthijssens F., McCarthy K., Barata J.T., Speleman F., Taghon T., Gutierrez A., Van Vlierberghe P., Haas W., Blackburn J.S, & Langenau D.M. (2020). PRL3 enhances T cell acute lymphoblastic leukemia growth through suppressing T cell signaling pathways and apoptosis. Leukemia, 35(3), 679-690.
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2

Western Blot Analysis of Signaling Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blot analysis used the following rabbit anti-human antibodies: PRL3 (GTX 100600, VWR # 89307–822, Radnor, Pennsylvania), VAV1 (Cell Signaling Technology #2502, Danvers, Massachusetts), Pan pY (Cell Signaling Technology #8954), and GAPDH (Cell Signaling Technology #2118), CBL (Cell Signaling Technology # 2747), CBL pY674 (Abcam # ab76536, Cambridge, United Kingdom), ITK (Cell Signaling Technology # 77215S), ITK pTyr512 (Life Technologies #PA564523), LAT pY255 ( Thermo Fisher Scientific # PIPA564800), LAT (Cell Signaling Technology # 9166S). Antibody binding was visualized with anti-rabbit HRP and developed by either Amersham ECL Prime reagent (GE Healthcare, Buckinghamshire, United Kingdom) or SuperSignal West Femto Chemiluminescent Substrate (Thermo Fisher Scientific) using a Fluor-S Multi-Imager (Bio-Rad, Hercules, California).
Garcia E.G., Veloso A., Oliveira M.L., Allen J.R., Loontiens S., Brunson D., Do D., Yan C., Morris R., Iyer S., Garcia S.P., Iftimia N., a L.W., Matthijssens F., McCarthy K., Barata J.T., Speleman F., Taghon T., Gutierrez A., Van Vlierberghe P., Haas W., Blackburn J.S, & Langenau D.M. (2020). PRL3 enhances T cell acute lymphoblastic leukemia growth through suppressing T cell signaling pathways and apoptosis. Leukemia, 35(3), 679-690.
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