HDPCs provided by PromoCell (Heidelberg, Germany) were purchased and maintained in a follicle growth medium kit (Promocell, Heidelberg, Germany) with 5% CO2 and at 37 °C. Passage 4 to 6 HDPCs were used in in vitro cultivation and all the experiments in this study. Allium hookeri used in this study were collected in March 2022 from a cultivation area located in the Pyeongchang region (Gangwon-do, Republic of Korea). The plants were washed with distilled water three times and air-dried at room temperature (in the shade). Fifty grams of dried A. hookeri was chopped and ground into a powder using a grinder (SMX-3500GN, Shinil Industrial Co., Ltd., Seoul, Republic of Korea). Then, 50 g of the ground A. hookeri powder was mixed with 900 mL of distilled hot water (80 °C) for 4 h. The obtained extracts were filtered through Whatman filter paper No. 1 (Whatman, Maidstone, UK) followed by ultrafiltration through a sterile 0.2 µm bottle-top vacuum filter (Corning, Corning, NY, USA). Using HPLC-HRMS and DPPH analyses, the extract was determined to have the main component, alliin (MedChemExpress, Monmouth Junction, NJ, USA), and a radical scavenging effect; therefore, these two methods were used to monitor the quality of the extracts.
Hdpcs
Manufactured by PromoCell
Sourced in Germany
HDPCs are a type of laboratory equipment used for the cultivation and maintenance of human dermal papilla cells. They provide a controlled environment for cell growth and experimentation.
Automatically generated - may contain errors
Lab products found in correlation
Bottle top vacuum filter, by Corning (1 mentions)
Whatman no 1 filter paper, by Cytiva (1 mentions)
Follicle growth medium kit, by PromoCell (1 mentions)
Alliin, by MedChemExpress (1 mentions)
Whatman, by Cytiva (1 mentions)
Follicle dermal papilla cell growth medium kit, by PromoCell (1 mentions)
Hdpcs, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Horscs, by ScienCell (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Basic fibroblast growth factor (bfgf), by Merck Group (1 mentions)
Adenosine, by Merck Group (1 mentions)
Supplementmix, by PromoCell (1 mentions)
Zm241385, by Bio-Techne (1 mentions)
Ly294002, by Bio-Techne (1 mentions)
Dihydrotestosterone dht, by Merck Group (1 mentions)
Akt inhibitor ly294002, by Cell Signaling Technology (1 mentions)
7 protocols using hdpcs
1
Extraction and Characterization of Allium hookeri
2
Cultivation and Propagation of Human Dermal Papilla Cells
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Human dermal papilla cells (hDPCs) (Promocell, Germany) were thawed and resuscitated and then mixed with high-glucose DMEM containing 15% FBS (15% conditioned DMEM). The mixture was cultured in an incubator (37 °C and 5% CO 2 ) for 24 h, and the culture medium was changed every 1-2 days. When the cells were approximately 80% confluent, they were passaged and frozen. The frozen cells were resuscitated and cultured, and hDPCs were digested at the second passage and used in the following assays.
3
Pangasius Skin Gelatin Hydrolysate Protocol
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LMWCP supplied by NEWTREE Co., Ltd., South Korea was prepared by spray drying the gelatin hydrolysate obtained by enzymatic degradation of gelatin derived from a skin of pangasius hypophthalmus using a protease and was standardized based on Gly-Pro-Hyp (3%) and tripeptide (≥15%) contents. hDPCs were purchased from PromoCell (Germany). The cells were maintained in an incubator with 5% CO2 at 37°C using a follicle dermal papilla cell growth medium kit (PromoCell) and subcultured upon reaching 70-80% confluency.
4
Culturing Human Dermal Papilla Cells
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We purchased hDPCs from Promocell (GmbH, Heidelberg, Germany) and cultured them as previously described [1 (link)]. The hDPCs were seeded at a density of 2 × 105 cells per well on 6-well culture plates in Dulbecco Modified Eagle Medium (DMEM, Gibco BRL, Life Technology, Karlsruhe, Germany) supplemented with 10% fetal bovine serum (FBS; Gibco BRL, Life Technology, Karlsruhe, Germany) and 1% penicillin/streptomycin (Gibco, BRL) for 24 h. The hDPCs in passages 3 to 4 were used for the experiments.
5
Culturing hDPCs and hORSCs for Experiments
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hDPCs were purchased from Promocell (Heidelberg, Germany) and hORSCs were purchased from ScienCell research laboratories (Carlsbad, CA, USA). hDPCs were cultured in a basal medium supplemented with Supplement Mix, which contains 4% fetal calf serum, 0.4% bovine pituitary extract, 1 ng/mL of basic fibroblast growth factor, and 5 μg/mL of insulin. hORSCs were cultured in a mesenchymal stem cell medium containing 5% FBS, 1% penicillin streptomycin and 1% MSCGS (Mesenchymal Stem Cell Growth Supplement) which is provided by the manufacturer. Cells were maintained in a humidified incubator at 37 °C with 5% CO2. hDPCs under the passage number 5 were used for experiments.
6
Adenosine Effects on Human Dermal Papilla Cells
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hDPCs were purchased from Promocell (Heidelberg, Germany) and cultured in basal medium supplemented with 4% fetal calf serum, 0.4% bovine pituitary extract, 1 ng/mL basic fibroblast growth factor, and 5 μg/mL insulin (Supplement Mix, Promocell). Cells were maintained in a humidified incubator at 37 °C, 5% CO2. adenosine stock solution (10 mM) was prepared just before each experiment. Before adenosine (Sigma-Aldrich, MO, USA) treatment, serum limitation was conducted by replacing the medium with fresh DMEM (Gibco, Waltham, MA, USA) supplemented with 1% FBS (Gibco, Waltham, MA, USA) and 1 ng/mL bFGF (Merck, Darmstadt, Germany) and culturing for 24 h to minimize the effects of serum and growth supplements. ZM241385, PBS603, KH7, H89, LY294002, and IWR1 were purchased from Tocris Bioscience (Bristol, UK).
7
Human Dermal Papilla Cell Culture
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Human dermal papilla cells (hDPCs, sourced from scalp of a 57-old female) were purchased from PromoCell (Heidelberg, Germany). hDPCs were cultured in Follicle Dermal Papilla Cell Growth Media (PromoCell) supplemented with provided mixture reagent at 37 °C in a humidified atmosphere of 5% CO2. Dihydrotestosterone (DHT) from Sigma-Aldrich (St. Louis, MO, USA). LY294002 (AKT inhibitor) were from Cell Signaling Technology (Beverly, MA, USA). TM30089 (DP2 antagonist) was from Caymen Chemical (Ann Arbor, MI, USA). For treatment, the reagents were dissolved in 100% methanol and DMSO to a concentration at 10 mM. Three to fourth-passage DPCs were used in each experiment.
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