Ftvii

BMSCs were digested and washed twice with Ca²⁺- and Mg²⁺- free Hanks Balanced Salt Solution (HBSS) (Gibco, NY, USA). After centrifugation, the cell precipitates were treated with fucosyltransferase VII (FTVII) reaction buffer system, containing the 60 mU/mL FTVII (Cusabio, WuHan, China), 20 mM HEPES (Sigma, MissouriState, USA), 1% human serum albumin (HSA, Sigma, MissouriState, USA) and 1 mM GDP-fucose (Sigma, MissouriState, USA) in HBSS. The mixture was then incubated in a 5% CO₂ incubator at 37℃ for 60 min, and mixed once every 15 min. The cells were then washed with HBSS containing 1% HSA and 20 mM HEPES and suspended in 1× phosphate-buffered saline (PBS) for later use. Cell viability was assessed by trypan blue staining.