Cells were seeded on polyacrylamide substrata and swapped to GF-free medium as described above. Cells were then treated with 20 ng/mL EGF-Alexa-488 (EGF-488) (Thermo Fisher), fixed with 4% paraformaldehyde, stained with Hoechst 33342 (Invitrogen), or immunostained for EGFR and E-cadherin. Samples were imaged by confocal microscopy using a 60× oil objective.
Egf alexa 488 egf 488
Manufactured by Thermo Fisher Scientific
EGF-Alexa-488 (EGF-488) is a fluorescently labeled epidermal growth factor (EGF) conjugate. It is used as a tool for fluorescence-based cellular and molecular biology research applications.
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Lab products found in correlation
2 protocols using egf alexa 488 egf 488
1
Quantifying EGFR Dynamics in Cells
2
Quantifying EGFR Dynamics in Cells
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Cells were seeded on polyacrylamide substrata and swapped to GF-free medium as described above. Cells were then treated with 20 ng/mL EGF-Alexa-488 (EGF-488) (Thermo Fisher), fixed with 4% paraformaldehyde, stained with Hoechst 33342 (Invitrogen), or immunostained for EGFR and E-cadherin. Samples were imaged by confocal microscopy using a 60× oil objective.
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