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Sds homogeneous 15 page

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The SDS-homogeneous 15% PAGE is a laboratory equipment used for the separation and analysis of proteins based on their molecular weight. It utilizes a polyacrylamide gel electrophoresis (PAGE) technique with sodium dodecyl sulfate (SDS) to denature and charge the proteins, allowing for their migration through the gel matrix.

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Lab products found in correlation

Trypsin, by Promega (1 mentions) Superdex 200 column, by GE Healthcare (1 mentions) Kta explorer, by GE Healthcare (1 mentions)

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SDS‐PAGE (2 citations)

2 protocols using sds homogeneous 15 page

1

Trypsin Proteolysis Time-Course Analysis

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WT and N184K G2l were dissolved at 1 mg/ml in PBS, pH 7.4. Trypsin (PROMEGA) was added to the sample at a concentration of 2 ng/μl at room temperature. Proteolysis samples (10, 20, 30, 60, 90, 120 minutes) were analysed by SDS-homogeneous 15% PAGE (GE Healthcare).
Bonì F., Milani M., Porcari R., Barbiroli A., Ricagno S, & de Rosa M. (2016). Molecular basis of a novel renal amyloidosis due to N184K gelsolin variant. Scientific Reports, 6, 33463.
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2

Size-Exclusion Chromatography of Trypsin-Digested TTR

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Size-exclusion chromatography of S52P TTR after digestion with trypsin (5 ng/μl) (Promega Trypsin Gold Mass Spectrometry Grade) was performed using a Superdex 200 column on the ÄKTA Explorer apparatus (GE Healthcare). The column was equilibrated and eluted at 0.5 ml/min with phosphate-buffered saline, pH 7.4 (PBS). The eluted peak was analysed by SDS–homogeneous 15% PAGE (GE Healthcare) under reducing conditions and mass spectrometry analysis after sample denaturation with 25% acetonitrile and 0.05% formic acid.
Marcoux J., Mangione P.P., Porcari R., Degiacomi M.T., Verona G., Taylor G.W., Giorgetti S., Raimondi S., Sanglier-Cianférani S., Benesch J.L., Cecconi C., Naqvi M.M., Gillmore J.D., Hawkins P.N., Stoppini M., Robinson C.V., Pepys M.B, & Bellotti V. (2015). A novel mechano-enzymatic cleavage mechanism underlies transthyretin amyloidogenesis. EMBO Molecular Medicine, 7(10), 1337-1349.
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