Tissue culture medium 199 (TCM 199) and fetal calf serum were purchased from Gibco BRL (Paisley, Scotland, UK). Follicle-stimulating hormone, formic acid (FA), bis-acrylamide and acetonitrile (ACN) were supplied by Sigma (St, Louis, MO, USA). Urea, iodoacet-amide (IAA), ammonium bicarbonate (NH4HCO3) were obtained from Amresco (Solon, OH, USA). Protease inhibitor complete tablets was purchased from Roche. iTRAQ-4plex Regent kit was obtained from Applied Biosystems (Forster City, CA, USA). Trypsin was from Promega (Madison, WI, USA). All the water was prepared using a MilliQ system (Bedford, MA, USA).
Tissue culture medium 199 tcm 199
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom, United States
Tissue culture medium 199 (TCM 199) is a widely used cell culture medium formulated to support the growth and maintenance of a variety of cell types. It provides a balanced mixture of essential nutrients, vitamins, and other components necessary for cell proliferation and survival.
Automatically generated - may contain errors
Lab products found in correlation
Urea, by Avantor (1 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions)
Follicle stimulating hormone, by Merck Group (1 mentions)
Bis acrylamide, by Merck Group (1 mentions)
Complete protease inhibitor tablet, by Roche (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Trypsin, by Promega (1 mentions)
2 protocols using tissue culture medium 199 tcm 199
1
Quantitative Proteomic Analysis of Cells
2
Porcine Oocyte In Vitro Maturation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Porcine cumulus-oocyte complexes (COCs) were aspirated from follicles (3–6
mm diameter) using a 10 mL syringe with an 18-gauge needle. COCs were washed in
Tyrode’s lactate (TL)-Hepes medium containing 0.1% (w/v) polyvinyl
alcohol (PVA), and cultured in 500 μL of in vitromaturation (IVM) medium at 39℃, 5% CO2 in air for 42–44
h. The IVM medium was Tissue Culture Medium 199 (TCM199; Gibco, Grand Island,
NY, USA) supplemented with 3.05 mM D-glucose, 0.91 mM Na-pyruvate, 0.57 mM
cysteine, 0.1% (w/v) PVA, 75 μg/mL penicillin G, 50 μg/mL
streptomycin, 0.01 IU/mL follicle stimulating hormone (FSH), 0.01 IU/mL
luteinizing hormone (LH), and 10 ng/mL epidermal growth factor (EGF).
mm diameter) using a 10 mL syringe with an 18-gauge needle. COCs were washed in
Tyrode’s lactate (TL)-Hepes medium containing 0.1% (w/v) polyvinyl
alcohol (PVA), and cultured in 500 μL of in vitromaturation (IVM) medium at 39℃, 5% CO2 in air for 42–44
h. The IVM medium was Tissue Culture Medium 199 (TCM199; Gibco, Grand Island,
NY, USA) supplemented with 3.05 mM D-glucose, 0.91 mM Na-pyruvate, 0.57 mM
cysteine, 0.1% (w/v) PVA, 75 μg/mL penicillin G, 50 μg/mL
streptomycin, 0.01 IU/mL follicle stimulating hormone (FSH), 0.01 IU/mL
luteinizing hormone (LH), and 10 ng/mL epidermal growth factor (EGF).
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