Certified human adherent colon cancer cell lines: Caco-2 (ATCC® HTB-37™), HCT 116 (ATCC® CCL-247™) and HT-29 (ATCC® HTB-38™) were purchased from ATCC (MD, USA). Cells were cultured in 75 cm2 cell culture flasks (Nunc™ EasYFlask™ Nunclon™ Delta Surface; Thermo Fisher Scientific, Waltham, MA, USA) at 37 °C in 95% air with 5% CO2 in CELCULTURE® CCL-170B-8 incubator (Esco, Singapore), in Dulbecco’s modified eagle medium (DMEM; Gibco, Thermo Fisher Scientific), supplemented with 10% fetal bovine serum (Gibco), 1% GlutaMAX™-I (Gibco), and with 1% stabilized antibiotic antimycotic solution (10,000 units penicillin/mL, 10 mg streptomycin/mL, 25 µg amphotericin B/mL; Sigma Aldrich, St. Louis, MO, USA). Medium was renewed in every 3 days. Cells were harvested with TrypLE™ Express (Gibco) after rinsing with Dulbecco’s Phosphate Buffered Saline (DPBS; Gibco). Cells were counted with Countess™ Automated Cell Counter (Invitrogen, Thermo Fisher Scientific, Waltham, CA, USA) after staining with 0.4% trypan blue solution (Invitrogen).
Celculture ccl 170b 8 incubator
Manufactured by Esco Lifesciences
Sourced in Singapore
The CELCULTURE® CCL-170B-8 incubator is a laboratory equipment designed to provide a controlled environment for cell culture applications. It maintains a stable temperature, humidity, and CO2 levels to support the growth and proliferation of cell lines.
Automatically generated - may contain errors
Lab products found in correlation
Trypan blue solution, by Thermo Fisher Scientific (2 mentions)
Countess automated cell counter, by Thermo Fisher Scientific (2 mentions)
Tryple express, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle medium, by Thermo Fisher Scientific (1 mentions)
Nunc easyflask nunclon delta surface, by Thermo Fisher Scientific (1 mentions)
Ht 29 atcc htb 38, by American Type Culture Collection (1 mentions)
Dulbecco s phosphate buffered saline dpbs, by Thermo Fisher Scientific (1 mentions)
Glutamax 1, by Thermo Fisher Scientific (1 mentions)
Amphotericin b, by Merck Group (1 mentions)
Penicillin, by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Dulbecco s phosphate buffered saline solution dpbs, by Thermo Fisher Scientific (1 mentions)
Htb 37, by American Type Culture Collection (1 mentions)
Amphotericin b, by Thermo Fisher Scientific (1 mentions)
Biowhittaker, by Lonza (1 mentions)
2 protocols using celculture ccl 170b 8 incubator
1
Cultivation of Colon Cancer Cell Lines
2
Culturing Colorectal Cancer Cell Lines
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The human colorectal adenocarcinoma Caco-2 cells (ATCC ® HTB-37 ™ ) and human colorectal carcinoma HCT 116 cells (ATCC ® CCL-247 ™ ) were cultured at 37°C in 95% air with 5% CO 2 in CELCULTURE ® CCL-170B-8 incubator (Esco, Singapore) with EMEM (Eagle's Minimum Essential Medium; BioWhittaker ® , Lonza, Basel, Switzerland), supplemented with 2 mmol/L L-glutamine, 10% (v/v) fetal bovine serum (FBS), 100 U/mL penicillin, 100 µg/mL streptomycin, and 0.25 µg/mL amphotericin B (Gibco, Thermo Fisher Scientific). The medium was renewed every 3 days. Cells were harvested with TrypLE ™ Express (Gibco, Thermo Fisher Scientific) after rinsing with Dulbecco's Phosphate Buffered Saline solution (DPBS; Gibco, Thermo Fisher Scientific). The cells were counted with Countess ™ Automated Cell Counter (Invitrogen) after staining with 0.4% trypan blue solution (Invitrogen).
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