For the analysis of monocyte populations in whole blood, 100 μl of EDTA blood was pipetted into Absolute Counting Tubes (Becton Dickinson) and incubated at room temperature for 20 min protected from the light with the following fluorochrome-conjugated monoclonal antibodies: anti-CD14-Alexa Fluor 647 (clone M5E2, Biolegend), anti-CD16-BV510 (clone 3G8, Biolegend), anti-CD66b-Alexa Fluor 700 (clone G10F5, Biolegend), anti-NKp46-FITC (clone 9E2, Biolegend), anti-CD11b-FITC (clone ICRF44, Biolegend), anti-CD3-FITC (clone UCHT1, Biolegend), anti-CD19-FITC (clone H1B19, Becton Dickinson), anti-HLA-DR-BUV395 (clone G46-6, Becton Dickinson), anti-CCR2-BV421 (clone 48,607, Becton Dickinson) and anti-CD45-BV786 (clone HI30, Becton Dickinson). After staining, the blood was incubated for 15 min at room temperature in the dark with 1-step fix/lyse solution (ThermoFisher). Stained fixed and lysed blood was stored at 4 °C in the dark before being run on an LSRFortessa™ with FACsDIVA software (Becton Dickinson) the next day. At least 5000 bead events were collected from each fully stained tube at the low acquiring speed and data was analysed using FlowJo software (TreeStar).
Anti nkp46 fitc clone 9e2
Manufactured by BioLegend
Anti-NKp46-FITC (clone 9E2) is a fluorochrome-conjugated monoclonal antibody that recognizes the NKp46 receptor expressed on natural killer (NK) cells. It can be used for the identification and enumeration of NK cells in flow cytometric analysis.
Automatically generated - may contain errors
Lab products found in correlation
Absolute counting tubes, by BD (2 mentions)
Anti cd14 alexa fluor 647 clone m5e2, by BioLegend (2 mentions)
Anti cd16 bv510 clone 3g8, by BioLegend (2 mentions)
Anti cd66b alexa fluor 700 clone g10f5, by BioLegend (2 mentions)
Anti cd11b fitc clone icrf44, by BioLegend (2 mentions)
Anti cd19 fitc clone h1b19, by BD (2 mentions)
Anti hla dr buv395 clone g46 6, by BD (2 mentions)
Anti ccr2 bv421 clone 48 607, by BD (2 mentions)
Anti cd45 bv786 clone hi30, by BD (2 mentions)
Lsrfortessa with facsdiva software, by BD (2 mentions)
Anti cd3 fitc clone ucht1, by BioLegend (2 mentions)
1 step fix lyse solution, by Thermo Fisher Scientific (2 mentions)
2 protocols using anti nkp46 fitc clone 9e2
1
Comprehensive Monocyte Profiling in Whole Blood
2
Comprehensive Monocyte Profiling in Whole Blood
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For the analysis of monocyte populations in whole blood, 100 μl of EDTA blood was pipetted into Absolute Counting Tubes (Becton Dickinson) and incubated at room temperature for 20 min protected from the light with the following fluorochrome-conjugated monoclonal antibodies: anti-CD14-Alexa Fluor 647 (clone M5E2, Biolegend), anti-CD16-BV510 (clone 3G8, Biolegend), anti-CD66b-Alexa Fluor 700 (clone G10F5, Biolegend), anti-NKp46-FITC (clone 9E2, Biolegend), anti-CD11b-FITC (clone ICRF44, Biolegend), anti-CD3-FITC (clone UCHT1, Biolegend), anti-CD19-FITC (clone H1B19, Becton Dickinson), anti-HLA-DR-BUV395 (clone G46-6, Becton Dickinson), anti-CCR2-BV421 (clone 48,607, Becton Dickinson) and anti-CD45-BV786 (clone HI30, Becton Dickinson). After staining, the blood was incubated for 15 min at room temperature in the dark with 1-step fix/lyse solution (ThermoFisher). Stained fixed and lysed blood was stored at 4 °C in the dark before being run on an LSRFortessa™ with FACsDIVA software (Becton Dickinson) the next day. At least 5000 bead events were collected from each fully stained tube at the low acquiring speed and data was analysed using FlowJo software (TreeStar).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!