15-mer peptides covering hemagglutinin (GenBank entry ACP41953.1) and neuraminidase (YP_009118627.1) of influenza (A/California/07/2009 (H1N1)), and nucleoprotein (ADE29096.1) of influenza (A/reassortant/NYMC X-179A (California/07/2009 × NYMC X-157)(H1N1)) with 12 amino acid overlap were produced. 2 × 5 15-mer peptides with 12 amino acid overlap, covering 2 viral peptide mimotopes identified in POMT1 (AAH65268.1) and SNTG1 (AAI04830.1), respectively, were also produced (all peptides from New England Peptides, Gardner, MA, USA). Peptides that failed in-house quality control by mass spectrometry were excluded (0–5.4% of viral peptides). Peptides were dissolved in endotoxin-free water/DMSO (50%; both from Sigma-Aldrich, Schnelldorf, Germany), and stored as 25 mM stock solutions at −70 °C, until the day of use. Recombinant influenza A H1N1 (A/California/07/2009) hemagglutinin (cat#11085-V08H), (A/California/04/2009) neuraminidase (cat#11058-VNAHC or cat#11058-V01H), and (A/California/07/2009) or (A/Puerto Rico/8/34/Mount Sinai) nucleoprotein (cat#40205-V08B and cat#11675-V08B) were purchased from Sino Biological, Beijing, China. Endotoxin-free, recombinant human protein-O-mannosyltransferase 1 and syntrophin gamma 1 were from Origene, Rockville, Maryland, and endotoxin-free ovalbumin from Hyglos, Bernried, Germany.
Recombinant influenza a h1n1
Manufactured by Sino Biological
Sourced in China
Recombinant influenza A H1N1 is a lab equipment product developed by Sino Biological. It is a strain of the influenza A virus subtype H1N1.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using recombinant influenza a h1n1
1
Peptide Production for Influenza Epitope Study
2
Indirect ELISA for Detecting Anti-H1N1 Antibodies
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Recombinant influenza A H1N1 (A/Puerto Rico/8/1934) hemagglutinin and influenza A H1N1 (A/Puerto Rico/8/1934) hemagglutinin-specific mouse monoclonal antibody were purchased from Sino Biological (Beijing, China).
For indirect enzyme-linked immunosorbent assay (ELISA), 1 µg of recombinant hemagglutinin protein was coated onto a 96-well microplate in 100 mM carbonate-bicarbonate buffer (pH 9.6) and blocked with OptEIA Assay Diluent (55,213, BD Biosciences, Franklin Lakes, NJ, USA) for 1 h at room temperature. Following five washes with 1× PBS, plasma from PR8 virus-infected mice was added and incubated for 2 h at room temperature. Unbound antibodies were eliminated by washing five times with 1× PBS containing Tween 20. The signal was developed based on the enzymatic reaction of horseradish peroxidase-conjugated anti-mouse immunoglobulin G (IgG) with 3,3′,5,5′-Tetramethylbenzidine (TMB) substrate (555,214, BD Biosciences). The absorbance at 450 nm was assessed and the background absorbance at 570 nm was measured.
For indirect enzyme-linked immunosorbent assay (ELISA), 1 µg of recombinant hemagglutinin protein was coated onto a 96-well microplate in 100 mM carbonate-bicarbonate buffer (pH 9.6) and blocked with OptEIA Assay Diluent (55,213, BD Biosciences, Franklin Lakes, NJ, USA) for 1 h at room temperature. Following five washes with 1× PBS, plasma from PR8 virus-infected mice was added and incubated for 2 h at room temperature. Unbound antibodies were eliminated by washing five times with 1× PBS containing Tween 20. The signal was developed based on the enzymatic reaction of horseradish peroxidase-conjugated anti-mouse immunoglobulin G (IgG) with 3,3′,5,5′-Tetramethylbenzidine (TMB) substrate (555,214, BD Biosciences). The absorbance at 450 nm was assessed and the background absorbance at 570 nm was measured.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!