Catecholaminergic neurotoxin 6-OHDA was injected unilaterally into the left dorsal striatum (AP=+1.0, ML=+2.7, DV=-4.0 mm relative to the bregma, according to the rat brain atlas [30 ] of male Wistar rats in a stereotaxic surgery under isoflurane (Vetflurane® 1000 mg/g, Virbac, France) anaesthesia [31 (link)]. Rats were fixed on a stereotaxic frame (Stoelting, USA) using ear bars and incisor bar. Lidocaine-adrenalin-solution (10 mg/ml; Orion Pharma, Finland) was injected between the skull and the scalp for local anaesthesia and to prevent bleeding. The skull was exposed, and a burr hole was made using a high-speed drill. An electronic injector (Quintessential stereotactic injector, Stoelting) and a 10 μl microsyringe (Hamilton Company, USA) were used to deliver 16 μg of 6-OHDA (Sigma-Aldrich, Germany), calculated as free base and dissolved in ice-cold saline with 0.02% ascorbic acid. Injection rate was set to 1.0 μl/min and injection volume to 4 μl. At the completion of the injection, the needle was kept in place for 4 minutes and then slowly withdrawn. Desipramine (15 mg/kg i.p.; calculated as free base; Sigma-Aldrich) was administered 30 minutes before the 6-OHDA injection to prevent the uptake of 6-OHDA into noradrenergic and serotonergic nerve terminals.
Vetflurane 1000
Manufactured by Virbac
Sourced in France
Vetflurane® 1000 is a veterinary anesthetic agent used for the induction and maintenance of general anesthesia in cats and dogs. It is a volatile liquid formulation containing isoflurane as the active ingredient.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using vetflurane 1000
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6-OHDA Unilateral Striatal Lesion
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6-OHDA Unilateral Striatal Lesion
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Catecholaminergic neurotoxin 6-OHDA was injected unilaterally into the left dorsal striatum (AP=+1.0, ML=+2.7, DV=-4.0 mm relative to the bregma, according to the rat brain atlas [30 ] of male Wistar rats in a stereotaxic surgery under isoflurane (Vetflurane® 1000 mg/g, Virbac, France) anaesthesia [31 (link)]. Rats were fixed on a stereotaxic frame (Stoelting, USA) using ear bars and incisor bar. Lidocaine-adrenalin-solution (10 mg/ml; Orion Pharma, Finland) was injected between the skull and the scalp for local anaesthesia and to prevent bleeding. The skull was exposed, and a burr hole was made using a high-speed drill. An electronic injector (Quintessential stereotactic injector, Stoelting) and a 10 μl microsyringe (Hamilton Company, USA) were used to deliver 16 μg of 6-OHDA (Sigma-Aldrich, Germany), calculated as free base and dissolved in ice-cold saline with 0.02% ascorbic acid. Injection rate was set to 1.0 μl/min and injection volume to 4 μl. At the completion of the injection, the needle was kept in place for 4 minutes and then slowly withdrawn. Desipramine (15 mg/kg i.p.; calculated as free base; Sigma-Aldrich) was administered 30 minutes before the 6-OHDA injection to prevent the uptake of 6-OHDA into noradrenergic and serotonergic nerve terminals.
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