Anti h3 9715

Anti-OGFOD1 (HPA003215) and anti-ACTB (A2228) were purchased from Sigma; anti-HA (MMS-101P) was obtained from Covance; anti-LIN54 (A303-799A) was purchased from Bethyl Labs; anti-LIN9 (ab62329), anti-H3K36me3 (ab9050-100), and anti-H4K20me1 (ab9051) /2 (ab9052) /3 (ab9053) were acquired from Abcam; anti-H4 (39269) was purchased from Active Motif; anti-H3 (#9715) was purchased from Cell Signaling; anti H3K27me3 (ABE44) was obtained from Millipore; anti E2F1 (sc-193), anti-E2F4 (sc-866), anti B-MYB (sc-724), anti-NF-YA (sc-10779), anti-P130 (sc-317), anti-FOXM1 (sc-502), anti-CCNA2 (sc-751), and anti-CCNB1 (sc-752) were purchased from Santa Cruz Biotechnology; and anti-AURKA (610938) and anti-AURKB (611092) were purchased from BD Transduction Labs.

Antibodies were obtained from the following commercial sources: anti-β-actin antibody (AC-15, A5441) was purchased from Sigma-Aldrich; the anti-caspase-3 (#9662), anti-caspase-9 (#9502), anti-caspase-8 (#9746), anti-PARP (#9542), anti-c-Myc (#5605), anti-HSP90 (#4874), anti-Smad4 (#9515) and anti-H3 (#9715) antibodies were purchased from Cell Signaling Technology (Tokyo, Japan); the anti-phospho-Smad1/Smad5/Smad8 antibodies recognizing phosphorylated Ser463/465 were purchased from Millipore (MA, USA); and the anti-phospho-Ser62-c-Myc (ab51156) and anti-phospho-Thr58-c-Myc (ab135558) antibodies were purchased from Abcam (Cambridge, MA). After treatment, the cells were washed once with cold PBS, the cell pellets were treated with RIPA buffer, and the total protein was isolated. Protein (10 μg) was resolved in polyacrylamide gels and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore). The membranes were probed with the primary antibody described above followed by a horseradish peroxidase-linked secondary antibody (GE Healthcare Japan). The signals were detected using Chemi-Lumi One (Nacalai Tesque) with Image Quant LAS 4000 mini software (GE Healthcare, Buckinghamshire, UK). The band density was analyzed using the NIH ImageJ software.