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Minidawn instrument

Manufactured by Wyatt Technology
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Sourced in United States

The MiniDawn instrument is a multi-angle light scattering (MALS) detector that measures the absolute molar mass and size of macromolecules and nanoparticles in solution. It is designed for online use with liquid chromatography and field-flow fractionation systems.

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Lab products found in correlation

Astra 6, by Wyatt Technology (2 mentions) Size exclusion superose 12 column, by GE Healthcare (2 mentions)

Spelling variants of this product

MiniDAWN TREOS MALS instrument (13 citations) MiniDAWN TREOS instrument (6 citations) Wyatt miniDAWN TREOS instrument (2 citations) Wyatt MiniDAWN TREOS MALS instrument (2 citations)

2 protocols using minidawn instrument

1

Molecular Mass Determination by MALLS

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Molecular mass determination was performed by multiple-angle laser light scattering (MALLS) using a miniDawn instrument (Wyatt Technology, Santa Barbara, CA, USA) for static measurements, in conjunction with a size-exclusion Superose-12 column (GE Healthcare). The protein concentration was 260 μM, and the elution buffer consisted of 20 mM Tris–HCl and either 50 mM or 200 mM NaCl, pH 7.0. The experiment was carried out at room temperature (25 °C) with a flow rate of 0.4 mL/min. Data analysis was conducted using the Astra 6.0 software (Wyatt Technology).
Doni D., Cavallari E., Noguera M.E., Gentili H.G., Cavion F., Parisi G., Fornasari M.S., Sartori G., Santos J., Bellanda M., Carbonera D., Costantini P, & Bortolus M. (2024). Searching for Frataxin Function: Exploring the Analogy with Nqo15, the Frataxin-like Protein of Respiratory Complex I from Thermus thermophilus. International Journal of Molecular Sciences, 25(3), 1912.
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2

Aggregation State Analysis by SLS

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The aggregation state was investigated by static light scattering using a miniDawn instrument (Wyatt Technology) coupled to a size-exclusion Superose-12 column (GE Healthcare). The elution buffer was 20 mM sodium acetate, 100 mM NaCl, pH 4.8. Protein concentration was 790 µM and the experiment was carried out at room temperature (~25 °C) and at a 0.4 ml/min flow rate. Data analysis was performed using the Astra 6.0 software (Wyatt Technology).
Noguera M.E., Roman E.A., Rigal J.B., Cousido-Siah A., Mitschler A., Podjarny A, & Santos J. (2015). Structural characterization of metal binding to a cold-adapted frataxin. Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry, 20(4).
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