All cell lines were obtained from the American Type Culture Collection (ATCC) and cultivated in a humidified incubator at 37 °C and 5% CO2. The human keratinocyte cell line HaCaT was cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma-Aldrich, D6419) supplemented with 10% fetal bovine serum (FBS, Sigma-Aldrich, F7524), 2 mM glutamine (Sigma-Aldrich, G7513), 0.1 mg/ml gentamicin (Gibco, 15710049), and 1.25 µg/ml fungizone (Sigma-Aldrich, A2942). For the lung carcinoma cell line A549 we used DMEM supplemented with 10% FBS and 2 mM glutamine, while the colorectal carcinoma cell line LS411N and the colorectal adenocarcinoma cell line DLD1 were cultivated in RPMI 1640 medium (Gibco, A1049101) supplemented with 10% FBS.
D6419
Manufactured by Merck Group
D6419 is a laboratory equipment product. It is a multi-purpose device designed for various laboratory applications. The core function of D6419 is to provide a reliable and versatile solution for laboratory tasks.
Automatically generated - may contain errors
2 protocols using d6419
1
Cultivation of Cell Lines for Research
2
Cell Culture Conditions for Diverse Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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All cell lines were obtained from the American Type Culture Collection (ATCC) and cultivated in a humidified incubator at 37°C and 5% CO2. The human keratinocyte cell line HaCaT was cultured in Dulbecco's modified Eagle's medium (DMEM, Sigma-Aldrich, D6419) supplemented with 10% fetal bovine serum (FBS, Sigma-Aldrich, F7524), 2 mM glutamine (Sigma-Aldrich, G7513), 0.1 mg/ml gentamicin (Gibco, 15710049), and 1.25 µg/ml fungizone (Sigma-Aldrich, A2942). For the lung carcinoma cell line A549 we used DMEM supplemented with 10% FBS and 2 mM glutamine, while the colorectal carcinoma cell line LS411N and the colorectal adenocarcinoma cell line DLD1 were cultivated in RPMI 1640 medium (Gibco, A1049101) supplemented with 10% FBS.
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