Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) activity and glucose content were analyzed using an automatic biochemical analyzer (Fuji Dri-Chem 3500, Fujifilm, Japan). The serum insulin content was measured using an insulin radioimmunoassay kit (EIKEN Chemical Co., Ltd., Tokyo, Japan), and serum leptin and adiponectin contents were analyzed using an ELISA kit (quantikine and immunoassay kit, R&D system, Minneapolis, MN, USA).
Insulin radioimmunoassay kit
Manufactured by Eiken Chemical
Sourced in Japan
The Insulin radioimmunoassay (RIA) kit is a laboratory assay used for the quantitative determination of insulin levels in biological samples. It utilizes the principle of competitive binding between radiolabeled and unlabeled insulin for a limited number of antibody binding sites.
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Lab products found in correlation
3 protocols using insulin radioimmunoassay kit
1
Serum Biochemical Markers in Metabolic Disorders
2
Plasma Insulin, GLP-1, and HbA1c Measurements
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Plasma insulin levels were measured using an insulin radioimmunoassay kit (Eiken Chemical Co., Ltd., Tokyo, Japan). Plasma GLP-1 levels were measured using a GLP-1 enzyme-linked immunosorbent assay (ELISA) kit (Shibayagiz, Gunma, Japan). The blood levels of HbA1c were measured by analyzing whole blood samples, using the MicroMat II Hemoglobin analyzer (Bio-Rad Laboratories, Hercules, CA, USA).
3
Serum Biochemical and Adipokine Analysis
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Glucose content and the activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) in the serum, were measured using the blood biochemical analyzer (Fuji Dri-Chem 3500). The levels of leptin and adiponectin in the serum secreted by adipose tissue were measured while using a leptin mouse/rat enzyme immunoassay (EIA) kit (Quantikine & Immuno Assay kit, R&D Systems, Minneapolis, MN, USA) and the adiponectin rat EIA (ALPCO Diagnostics, Salem, NH, USA), respectively, based on a sandwich-type enzyme-linked immunosorbent assay (ELISA), and then analyzed at 450 nm using a plate reader (Spectra Max 250; Molecular Devices, San Jose, CA, USA). Serum insulin levels were measured using an insulin radioimmunoassay kit (Eiken Chemical Co., Ltd., Tokyo, Japan).
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