Ncounter dxprep station

Manufactured by NanoString

The NCounter DxPrep Station is a lab equipment product designed for automated sample preparation for the NanoString nCounter Analysis System. The core function of the DxPrep Station is to prepare and process samples for analysis on the nCounter platform.

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Lab products found in correlation

Ncounter dx digital analyzer, by NanoString (2 mentions) Ncounter master kit, by NanoString (1 mentions) Paxgene blood rna kit, by Qiagen (1 mentions) Rneasy mini kit, by Qiagen (1 mentions) 2100 bioanalyzer, by Agilent Technologies (1 mentions) Agilent 2100 bioanalyzer, by Agilent Technologies (1 mentions)

2 protocols using ncounter dxprep station

Nanostring Transcriptomic Profiling of Tumor RNA

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Total RNA was purified from excised tumors using the RNeasy Mini kit (Qiagen). RNA from whole blood was was isolated using the Paxgene blood RNA kit (Qiagen). RNA quality was assessed using Bioanalyzer 2100 (Agilent) and final concentration determined by Qubit (Life Technologies). For nanostring analysis, 100 ng of purified RNA was added to 3 μL of Reporter CodeSet and 2 μL Capture ProbeSet using an nCounter master kit as recommended (NanoString Technologies). Samples were processed the following day using the nCounter DxPrep Station and nCounter Dx Digital Analyzer (NanoString Technologies). Data was analyzed using nSolver 2.6 software. Heat maps were created using Cluster 3.0 and Java TreeView-1.1.6r4.

McCaw T.R., Li M., Starenki D., Cooper S.J., Liu M., Meza-Perez S., Arend R.C., Buchsbaum D.J., Forero A, & Randall T.D. (2018). The expression of MHC class II molecules on murine breast tumors delays T cell exhaustion, expands the T cell repertoire and slows tumor growth. Cancer immunology, immunotherapy : CII, 68(2), 175-188.

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miRNA expression profiling via nCounter

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RNA was extracted using mirVana™ miRNA isolation kit. The quality of the total RNA was verified by an Agilent 2100 Bioanalyzer. miRNA array profiling was processed as recommended by the manufacturer (NanoString Technologies Inc). The samples were processed on an nCounter Dx Prep Station and counted using an nCounter Dx Digital Analyzer (NanoString Technologies Inc). Differences in miRNA expression were assessed using the nSolver program (version 3.0).

Nam H., Kundu A., Brinkley G.J., Chandrashekar D.S., Kirkman R.L., Chakravarthi B.V., Orlandella R.M., Norian L.A., Sonpavde G., Ghatalia P., Fei F., Wei S., Varambally S, & Sudarshan S. (2020). PGC1α suppresses kidney cancer progression by inhibiting collagen-induced SNAIL expression. Matrix biology : journal of the International Society for Matrix Biology, 89, 43-58.

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