Ecl chemidoc imaging system

Manufactured by Bio-Rad

The ECL Chemidoc imaging system is a laboratory equipment designed for the detection and analysis of chemiluminescent signals. It provides a reliable and sensitive method for visualizing and quantifying various biomolecules, such as proteins and nucleic acids, after separation by gel electrophoresis or blotting techniques.

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Lab products found in correlation

Pvdf membrane, by Cytiva (1 mentions) Ecl detection kit, by Cytiva (1 mentions) M per, by Thermo Fisher Scientific (1 mentions) Pvdf membrane, by Merck Group (1 mentions)

Close spelling variants of this product

ChemiDoc Imaging System ECL imaging system ECL Chemidoc Imaging Chemidoc ChemiDoc system ECL system Bio-Rad Imaging Systems ChemiDoc

2 protocols using ecl chemidoc imaging system

Western Blot Analysis of PfHtrA2-HA-glmS in P. falciparum

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Western blot analysis was carried out to assess the expression of PfHtrA2-HA-glmS in the transgenic P. falciparum blood-stage parasites. Briefly, parasites were lysed by 0.15% saponin, the supernatant and washed pellets were separately suspended in Laemmli buffer, boiled, centrifuged, and the supernatant obtained was separated on 12% SDS-PAGE. The fractionated proteins were transferred from the gel onto the PVDF membrane (Amersham, Piscataway, NJ, USA) and blocked-in blocking buffer (1 × PBS, 0.1% Tween-20, 5% skimmed milk powder) for 2 h. The blot was washed and incubated for 3 h with primary antibody [Rabbit anti-HtrA2 (1:500); rabbit anti-BiP (1:10000); rat anti-HA (1:1000)] diluted in dilution buffer (1 × PBS, 0.1% Tween-20, and 1% milk powder). Subsequently, the blot was washed and incubated for 1 h with an appropriate secondary antibody (anti-rabbit, anti-rat, or anti-mouse, 1:20000) conjugated to HRP. Bands were visualized by using an ECL detection kit (Amersham) with Biorad ECL Chemidoc imaging system.

Singh S., Datta G., Jain S., Thakur V., Arora P., Muneer A., Asad M., Ali S, & Mohmmed A. (2022). Dual role of an essential HtrA2/Omi protease in the human malaria parasite: Maintenance of mitochondrial homeostasis and induction of apoptosis-like cell death under cellular stress. PLOS Pathogens, 18(10), e1010932.

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Western Blot Protein Analysis Protocol

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Cells were lysed in mammalian protein extraction buffer (M-PER, Thermo Fisher) with protease/phosphatase inhibitor cocktail. The protein concentration was measured using a Pierce Coomassie (Bradford) protein assay. Protein samples was prepared in 4 × NuPAGE LDS sample buffer with 1.25% (v/v) 2-mercaptoethanol and denatured at 90 °C for 5 min. Equal amounts of protein samples were separated on a 4–12% SDS-PAGE Bis-Tris gel (Thermo Fisher) and transferred to PVDF membranes (MilliporeSigma) on a semidry blotting system (GenScript, Piscataway, NJ). Equal protein loading was immediately evaluated with Coomassie blue staining (staining solution: 0.1% Brilliant Blue, 50% methanol and 10% glacial acetic acid) after transfer. The PVDF membrane was then blocked with 2% (w/v) BSA in TBS with Tween-20 (TBST). Next, the membranes were incubated with primary antibodies diluted with TBST and 1% (w/v) BSA overnight at 4 °C and subsequently incubated with HRP-linked secondary antibodies for 1 h at RT. The results were visualized with HRP substrate reagent and detected with an ECL/ChemiDoc imaging system (Bio-Rad, Hercules, CA).

Lee J.J., Ishihara K., Notomi S., Efstathiou N.E., Ueta T., Maidana D., Chen X., Iesato Y., Caligiana A, & Vavvas D.G. (2019). Lysosome-associated membrane protein-2 deficiency increases the risk of reactive oxygen species-induced ferroptosis in retinal pigment epithelial cells. Biochemical and biophysical research communications, 521(2), 414-419.

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