The cell proliferation assay was performed using a Cell Counting Kit-8 kit (Vazyme Biotech, Nanjing) according to the manufacturer's instructions. The absorbance at 450nm was recorded and analyzed. For cell apoptosis assay, the indicated cells were stained with Annexin V-FITC/PI cell apoptosis detection kit (BD Pharmingen, San Diego, CA, USA) and were analyzed with a flow cytometer (BD FACSCalibur, San Jose, CA, USA)
Cell counting kit 8 kit
Manufactured by Vazyme
Sourced in China
The Cell Counting Kit-8 is a colorimetric assay for the determination of viable cell number. The kit utilizes the highly water-soluble tetrazolium salt WST-8, which is reduced by dehydrogenases in viable cells to produce a yellow-colored formazan dye. The amount of the formazan dye generated is directly proportional to the number of living cells in the culture.
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Lab products found in correlation
3 protocols using cell counting kit 8 kit
1
Cell Proliferation and Apoptosis Assay
2
BMSC Viability Assay with OPC
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BMSCs were seeded into 96-well plates (the cell density at 5 × 103 per well). BMSCs were incubated with different concentration of OPC (0, 2.5, 5, 10, 20, 40, and 80 µmol/L) for 24h, or were incubated with 10 µmol/L OPC for different times (0, 6, 12, 24, and 48 h). Cell viability of BMSCs was measured using a Cell Counting Kit-8 kit (Nanjing Vazyme Biotech Co., Ltd., Nanjing, Jiangsu, China) according to the correspondingly manufacturers’ instructions.
3
Antiproliferative Effects of Avasimibe and Fatostatin in Gastric Cancer
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The antiproliferative effect of avasimibe and fatostatin (MedChemExpress, Shanghai, China) was evaluated with a Cell Counting Kit-8 kit (CCK-8, Vazyme, Nanjing, China). One day before avasimibe treatment, GC cells were seeded in 96-well plates (5,000 cells/well). The cells were treated with avasimibe for 24 h or 48 h. After the indicated time, 10 µL per well of CCK-8 solution was added and incubated at 37°C for 1 h. Absorbance was recorded at 450 nm, and five independent assays were carried out.
For the plate colony formation assay, GC cells were seeded in 6-well plates or 12-well plates (1,000 cells/well in 6-well plates and 500 cells/well in 12-well plates) and incubated for 10–14 days. The medium with or without avasimibe was changed every other day. Then, the cells were fixed with 4% paraformaldehyde for 15 min and stained with crystal violet for 1 h. Images were acquired with a digital camera, and three independent assays were carried out.
For the plate colony formation assay, GC cells were seeded in 6-well plates or 12-well plates (1,000 cells/well in 6-well plates and 500 cells/well in 12-well plates) and incubated for 10–14 days. The medium with or without avasimibe was changed every other day. Then, the cells were fixed with 4% paraformaldehyde for 15 min and stained with crystal violet for 1 h. Images were acquired with a digital camera, and three independent assays were carried out.
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