Tissue samples were collected and rinsed with sterile saline. After being xed in 10% neutral buffered formaldehyde for 72 hours, tissues were embedded in para n. Sections of 4 µm thickness were obtained from para n blocks on slides by using a microtome (Leica SM 2000, Germany). The sections were depara nized using xylene and rehydrated using a graded ethanol series. The rehydrated sections were then stained with hematoxylin and eosin (H&E), viewed under a computerized photo-light microscope (Leica DM4000B, Germany) and and LAS software program (Leica), coded for blind examination.
Las software program
Manufactured by Leica
Sourced in Germany
The LAS software program is a comprehensive digital imaging and analysis solution for microscopy applications. It serves as the control interface for Leica's range of microscope systems, enabling users to capture, process, and analyze high-quality digital images. The software provides essential tools for image acquisition, image processing, and data management, supporting a wide variety of microscopy techniques and applications.
Automatically generated - may contain errors
2 protocols using las software program
1
Tissue Histology Processing and Analysis
2
Histological Analysis of Epididymis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Epididymis tissue samples were fixed in 10% neutral formaldehyde for seventy-two hours. After washing in tap water, the samples were dehydrated through ascending alcohols and cleared in xylene, then embedded in paraffin. Hematoxylin-Eosin was performed on 4 μm thick sections. The histomorphological changes were evaluated in epididymis using a photographic light microscope (DM4000B Image Analyze System; Leica, Wetzlar, Germany), a Leica DFC280 plus camera and an LAS software program (Leica).
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