The largest database of trusted experimental protocols

4 protocols using agonistic anti cd137 mab

1

Evaluation of Anti-CD137 Immunotherapy

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNAlater® was purchased from Ambion, USA. TRIzol was purchased from Invitrogen, USA. DEPC was purchased from Bio Basic Inc, Canada. The SYBR® PrimeScript® RT-PCR Kit was purchased from TaKaRa, Japan for two-step RT-PCR. PCR primers were designed by TaKaRa, Japan and synthesized by Yingjun Biotechnology Co., Ltd, China. An anti-CD137 rabbit mAb (#34549) used for IHC and IF and was purchased Cell Signaling Technology (CST, USA). An IHC detection reagent (HRP, rabbit, #8114) was purchased from CST, USA. An agonistic anti-CD137 mAb (#79097) was purchased from BPS Bioscience, USA. An anti-Foxp3 rabbit mAb (#12653) used for IHC was purchased from CST, USA. Anti-CD8 mouse antibody (#66868-1-Ig) for IHC and IF was purchased from the Proteintech group, China. MojoSort™ Magnet, MojoSort™ Human CD8 Nanobeads and MojoSort™ Human CD8 Cell Isolation Kit were purchased from BioLegend, USA. An NF-κB p65 rabbit mAb (#8242) for flow cytometry and IF was purchased from CST, USA. An anti-cytokeratin mouse mAb (#ab756) used for IHC was purchased from Abcam, England. A purified anti-human CD3 mAb (OKT3, #317326) for cell incubation and anti-CD45-PerCP (#368506), anti-CD3-FITC (#300406), anti-CD8-APC (#301014) and anti-CD137-APC (#309809) antibodies for flow cytometry were purchased from BioLegend, USA.
+ Open protocol
+ Expand
2

CD137 Expression Analysis in Tumor Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNAlater® was purchased from Ambion. TRIzol was purchased from Invitrogen. DEPC was purchased from Bio Basic Inc. The SYBR® PrimeScript® RT-PCR Kit was purchased from TaKaRa for two-step RT-PCR. PCR primers were designed by TaKaRa and synthesized by Yingjun Biotechnology Co., Ltd. An anti-CD137 rabbit mAb (#34549) used for IHC and IF and was purchased Cell Signaling Technology (CST, USA). An IHC detection reagent (HRP, rabbit, #8114) was purchased from CST. An agonistic anti-CD137 mAb (#79097) was purchased from BPS Bioscience. An anti-Foxp3 rabbit mAb (#12653) used for IHC was purchased from CST. Anti-CD8 mouse antibody (#66868-1-Ig) for IHC and IF was purchased from the Proteintech group. MojoSort TM Magnet, MojoSort TM Human CD8 Nanobeads and MojoSort TM Human CD8 Cell Isolation Kit were purchased from BioLegend. An NF-κB p65 rabbit mAb (#8242) for ow cytometry and IF was purchased from CST. An anti-cytokeratin mouse mAb (#ab756) used for IHC was purchased from Abcam. A puri ed anti-human CD3 mAb (OKT3, #317326) for cell incubation and anti-CD45-PerCP (#368506), anti-CD3-FITC (#300406), anti-CD8-APC (#301014) and anti-CD137-APC (#309809) antibodies for ow cytometry were purchased from BioLegend.
+ Open protocol
+ Expand
3

Detailed Immunology Reagents Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNAlater® was purchased from Ambion, USA. TRIzol was purchased from Invitrogen, USA. DEPC was purchased from Bio Basic Inc, Canada. The SYBR® PrimeScript® RT-PCR Kit was purchased from TaKaRa, Japan for two-step RT-PCR. PCR primers were designed by TaKaRa, Japan and synthesized by Yingjun Biotechnology Co., Ltd, China. An anti-CD137 rabbit mAb (#34549) used for IHC and IF and was purchased Cell Signaling Technology (CST, USA). An IHC detection reagent (HRP, rabbit, #8114) was purchased from CST, USA. An agonistic anti-CD137 mAb (#79097) was purchased from BPS Bioscience, USA. An anti-Foxp3 rabbit mAb (#12653) used for IHC was purchased from CST, USA. Anti-CD8 mouse antibody (#66868-1-Ig) for IHC and IF was purchased from the Proteintech group, China. MojoSort TM Magnet, MojoSort TM Human CD8 Nanobeads and MojoSort TM Human CD8 Cell Isolation Kit were purchased from BioLegend, USA. An NF-κB p65 rabbit mAb (#8242) for ow cytometry and IF was purchased from CST, USA. An anti-cytokeratin mouse mAb (#ab756) used for IHC was purchased from Abcam, England. A puri ed anti-human CD3 mAb (OKT3, #317326) for cell incubation and anti-CD45-PerCP (#368506), anti-CD3-FITC (#300406), anti-CD8-APC (#301014) and anti-CD137-APC (#309809) antibodies for ow cytometry were purchased from BioLegend, USA.
+ Open protocol
+ Expand
4

Detailed Immunology Reagents Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNAlater® was purchased from Ambion, USA. TRIzol was purchased from Invitrogen, USA. DEPC was purchased from Bio Basic Inc, Canada. The SYBR® PrimeScript® RT-PCR Kit was purchased from TaKaRa, Japan for two-step RT-PCR. PCR primers were designed by TaKaRa, Japan and synthesized by Yingjun Biotechnology Co., Ltd, China. An anti-CD137 rabbit mAb (#34549) used for IHC and IF and was purchased Cell Signaling Technology (CST, USA). An IHC detection reagent (HRP, rabbit, #8114) was purchased from CST, USA. An agonistic anti-CD137 mAb (#79097) was purchased from BPS Bioscience, USA. An anti-Foxp3 rabbit mAb (#12653) used for IHC was purchased from CST, USA. Anti-CD8 mouse antibody (#66868-1-Ig) for IHC and IF was purchased from the Proteintech group, China. MojoSort TM Magnet, MojoSort TM Human CD8 Nanobeads and MojoSort TM Human CD8 Cell Isolation Kit were purchased from BioLegend, USA. An NF-κB p65 rabbit mAb (#8242) for ow cytometry and IF was purchased from CST, USA. An anti-cytokeratin mouse mAb (#ab756) used for IHC was purchased from Abcam, England. A puri ed anti-human CD3 mAb (OKT3, #317326) for cell incubation and anti-CD45-PerCP (#368506), anti-CD3-FITC (#300406), anti-CD8-APC (#301014) and anti-CD137-APC (#309809) antibodies for ow cytometry were purchased from BioLegend, USA.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!