AFM measurements were performed using an Asylum MFP-3D head and controller (Asylum Research, Santa Barbara, CA, United States), mounted on an Olympus inverted microscope. Height images were recorded in tapping mode and in liquid at room temperature. Typically, 512 × 512 points scans were taken at a scan rate of 1 Hz per line. Both trace and retrace images were recorded and compared.
Asylum mfp 3d head and controller
Manufactured by Oxford Instruments
Sourced in United States
The Asylum MFP-3D head and controller is a high-precision scanning probe microscope that allows for the investigation of surface topography and properties at the nanoscale. It provides a versatile platform for a range of scanning probe microscopy techniques, including atomic force microscopy (AFM), magnetic force microscopy (MFM), and piezoresponse force microscopy (PFM). The instrument is designed to deliver reliable and accurate data collection, enabling researchers to study the characteristics of materials and surfaces with high resolution.
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Lab products found in correlation
2 protocols using asylum mfp 3d head and controller
1
Atomic Force Microscopy Imaging in Liquid
2
Atomic Force Microscopy of Biological Samples
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AFM measurements were carried out with an Asylum MFP-3D head and controller (Asylum Research, Santa Barbara, CA, USA). The driver program MFP-3D Xop was written in IGOR Pro Software (Wavemetrics, Lake Oswego, OR, USA). For imaging bacteria, gold coated silicon nitride rectangular cantilevers were used with a typical spring constant of 0.03 N/m, tip radius around 30 nm (BL-RC150 VB, Olympus Optical Co. Ltd., Tokyo, Japan). In case of purple membrane patches another type of silicon nitride cantilevers (BL-AC40 TS, Olympus Optical Co. Ltd., Tokyo, Japan) were used with 0.09 N/m spring constant.
The spring constant for each cantilever was determined by thermal calibration (Hutter and Bechhoefer 1993; (link)Butt and Jaschke 1995; (link)Florin et al. 1995) (link). Measurements were carried out in tapping mode in liquid. Typically, 512*512 point scans were taken at 0.4 Hz scan rate. Both the trace and retrace images were recorded and compared. Experiments were repeated 3-5 times for both peptide treatments.
The spring constant for each cantilever was determined by thermal calibration (Hutter and Bechhoefer 1993; (link)Butt and Jaschke 1995; (link)Florin et al. 1995) (link). Measurements were carried out in tapping mode in liquid. Typically, 512*512 point scans were taken at 0.4 Hz scan rate. Both the trace and retrace images were recorded and compared. Experiments were repeated 3-5 times for both peptide treatments.
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