1000 hplc system

Molecular weight of polysaccharides was measured using high-performance gel permeation chromatography (HP-GPC) on a Waters 1000 HPLC system, with a Grace 3300 Evaporative light Scattering Detector, ELSD. Samples (10.0 mg) were dissolved in distilled water (10.0 ml), passed through a 0.45 µm filter and applied to a gel-filtration chromatographic column of Ultrahydrogel TM Linear (300 mm×7.8 mm. Waters., USA).
Deionized water was used as the flow phase at a flow rate of 0.5 ml/min. The temperature of the column was maintained at 30 o C and the injection volume was 10 µl. Preliminary calibration of the column was carried out using Dextran standards with different molecular weights.

The molecular weight of polysaccharide was measured using high-performance gel permeation chromatography (HPGPC) on a Waters 1000 HPLC system, using a Waters 2414 Refractive Index Detector. Samples (10.0 mg) were dissolved in distilled water (10.0 mL), passed through a 0.45 μm filter and applied to a gel-filtration chromatographic column of Ultrahydrogel™ Linear (300 mm × 7.8 mm, Waters, USA). Deionized water was used as a mobile phase at a flow rate of 0.5 mL/min. The temperature of the column was maintained at 30 o C and the injection volume was 10 μL. Preliminary calibration of the column was carried out using dextran standards with different molecular weights.