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2 3 didehydro 3 deoxythymidine

Manufactured by Merck Group
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2',3'-Didehydro-3'-deoxythymidine is a synthetic nucleoside analog commonly used as a research tool. It functions as a thymidine kinase substrate and can be utilized in various cell-based assays and experiments.

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Lab products found in correlation

Methyl viologen dichloride hydrate, by Merck Group (1 mentions) 3 azido 3 deoxythymidine, by Merck Group (1 mentions) 2 3 dideoxycytidine, by Merck Group (1 mentions) 2 3 dideoxyinosine, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Milli q integral water purification system, by Merck Group (1 mentions) Fiveeasy, by Mettler Toledo (1 mentions) Sodium dodecyl sulfate (sds), by Merck Group (1 mentions) Sodium phosphate dibasic dihydrate, by Merck Group (1 mentions) Sodium phosphate monobasic, by Merck Group (1 mentions) Potassium phosphate monobasic, by Merck Group (1 mentions) Thymidine, by Merck Group (1 mentions) Stavudine, by Merck Group (1 mentions)

2 protocols using 2 3 didehydro 3 deoxythymidine

1

Antiviral Compounds for Biological Research

Check if the same lab product or an alternative is used in the 5 most similar protocols
Dimethylsulfoxide (D4540 Sigma-aldrich), 2′,3′-Didehydro-3′-deoxythymidine (D1413 Sigma-aldrich), 3′-Azido-3′-deoxythymidine (A2169, Sigma-aldrich), 2'-3'-dideoxycytidine (D5782, Sigma-Aldrich), 2',3'-dideoxyinosine (D0162, Sigma-Aldrich), 3’-deoxy-3’-Fluorothymidine (361275, Sigma-Aldrich), Methyl viologen dichloride hydrate (856177 Sigma-Aldrich).
de Boer R., Smith R.L., De Vos W.H., Manders E.M., Brul S, & van der Spek H. (2015). Caenorhabditis elegans as a Model System for Studying Drug Induced Mitochondrial Toxicity. PLoS ONE, 10(5), e0126220.
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2

Phosphate-buffered SDS Preparation

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Sodium phosphate monobasic (NaH2PO4, ≥98%),
sodium phosphate dibasic dihydrate (Na2HPO4,
≥99.0%), sodium dodecyl sulfate (SDS, ≥98.5%), potassium phosphate
monobasic (KH2PO4, ≥99.0%), thymidine
(≥99%), 2′,3′-didehydro-3′-deoxythymidine
(stavudine, ≥98%), and nosyl chloride (97%) were purchased from
Sigma–Aldrich (Milwaukee, WI, USA). Zidovudine impurity B
(chlorothymidine, CLT, European Pharmacopoeia reference standard) was purchased
from LGC Standards (Wesel, Germany). 30mM phosphate buffer (PB) was prepared via
titration of 100 mM solutions of NaH2PO4 and
Na2HPO4 and monitored with a calibrated pH meter
(FiveEasy, Mettler Toledo, Columbus, OH, USA). SDS (100 mM) in 30 mM phosphate
buffer was prepared by dissolving SDS in 30 mM PB. Separation buffers were
prepared with, and reference standards were dissolved in, 18 MΩ-cm
deionized (DI) water obtained from a Milli-Q® Integral Water Purification
system (EMD Millipore, Billerica, MA, USA). All chemicals were of analytical
grade and were used as received.
Jones J., Ha N.S., Barajas A.G., Chatziioannou A.F, & van Dam R.M. (2020). Integration of high-resolution radiation detector for hybrid microchip electrophoresis (hybrid-MCE). Analytical chemistry, 92(4), 3483-3491.
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