Human hepatic stellate cells (LX2 cells), immortalized human derived cell line were kindly provided by Prof. Scott Friedman (Mount Sinai Hospital, New York). Murine H5V heart capillary endothelial cells were kindly provided by Dr. A. Vecchi (Mario Negri, Institute for Pharmacological Research, Milan, Italy). Murine NIH3T3 fibroblasts and murine RAW264.7 macrophages were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). LX2 cells were cultured in DMEM-Glutamax (Invitrogen, Carlsbad, CA) supplemented with 10% FBS and antibiotics (50 U/ml Penicillin and 50 μg/ml streptomycin, Sigma). RAW macrophages were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Lonza, Verviers, Belgium), and 3T3 and H5V cells were cultured in Dulbecco’s modified Eagle’s (DMEM) medium (Lonza) supplemented with 2 mM L-glutamine (Sigma, St. Louis, MO), 10% fetal bovine serum (FBS, Lonza) and antibiotics (50 U/ml Penicillin and 50 μg/ml streptomycin, Sigma).
Murine nih3t3 fibroblasts
Manufactured by American Type Culture Collection
Sourced in United States
Murine NIH3T3 fibroblasts are a well-established cell line derived from mouse embryonic fibroblasts. They are commonly used in cell biology research as a model system for studying various cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Dmem glutamax, by Thermo Fisher Scientific (1 mentions)
Raw264.7 macrophage, by American Type Culture Collection (1 mentions)
1640 medium, by Lonza (1 mentions)
Dulbecco s modified eagle s medium dmem, by Lonza (1 mentions)
Streptomycin, by Merck Group (1 mentions)
Penicillin, by Merck Group (1 mentions)
L glutamine, by Merck Group (1 mentions)
3 protocols using murine nih3t3 fibroblasts
1
Culturing Diverse Cell Lines
2
Culturing Murine NIH/3T3 Fibroblasts
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Murine NIH/3T3 fibroblasts was purchased from the American Type Culture Collection and cultured in growth medium, consisting of DMEM (4.5 g/L glucose) supplemented with 10% fetal bovine serum and 6 mmol/L l -glutamine.
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Culture of Murine NIH3T3 Fibroblasts
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Murine NIH3T3 fibroblasts were obtained from the American Type Culture Collection (ATCC). The cells were cultured in Dulbecco's modified Eagle's medium (DMEM), supplemented with 10% heat-inactivated fetal bovine serum (FBS), penicillin (100 unitsÁmL À1 ), streptomycin (100 lgÁmL À1 ), and 0.2% NaHCO 3 , at 37 °C in an atmosphere of 95% air and 5% CO 2 .
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