The PyMT and PyMT-N-cadherin mammary tumor cell lines were generated and characterized in 2007 as described (26 (link)). Briefly, primary mammary and metastatic tumor cell lines were derived from PyMT and PyMT-N-cad tumors or lung foci at 7 weeks post tumor onset by collagenase digestion, and plated in culture till they underwent crisis as detailed in (26 (link), 28 (link)). The MDA-MB-231 metastatic subline 3475 was obtained in 2009 from Dr. Joan Massague (Memorial Sloan-Kettering Cancer Center, NY) and was tested for lung colonizing activity. The BT549 cell line was obtained in 2012 from the American Type Culture Collection and was characterized as triple negative by the lack of ER, PR and HER2 expression. All cell lines were tested and found negative for mycoplasma.
Bt549 cell line
Manufactured by American Type Culture Collection
Sourced in United States
The BT549 cell line is a human breast cancer cell line derived from a primary ductal carcinoma. It is a well-established and widely used model for breast cancer research.
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2 protocols using bt549 cell line
1
Characterization of Mammary Tumor Cell Lines
2
Culturing and Transfecting BT-549 and MEF Cells
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The BT-549 cell line was purchased from the American Type Culture Collection (ATCC; Rockville, MD, USA). Preparation of wild-type and Prip-DKO MEFs was described previously25 (link). MEFs were immortalised by transfecting the simian virus 40 large T antigen. Cells were cultured under conventional growth conditions and transfected with plasmids or siRNA using the Lonza 4D-Nucleofector X Unit (Lonza, Basel, Switzerland) or Lipofectamine 3000 (Invitrogen).
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