HPLC grade water was acquired from Macron Fine Chemicals™ Avantor Performance Materials (Center Valley, PA). Formic acid (FA) and HPLC grade acetonitrile (ACN) were purchased from Fisher Scientific (Pittsburgh, PA). Sodium chloride (NaCl) and disodium phosphate (Na2HPO4) were acquired from Mallinckrodt Chemicals (St. Louis, MO). Ammonium bicarbonate (ABC), DL-dithiothreitol (DTT) and iodoacetamide (IAA) were obtained from Sigma-Aldrich (St. Louis, MO). Fetuin, α1-acid glycoprotein (AGP), porcine thyroglobulin (PTG) and pooled human blood serum (BS) were also obtained from Sigma-Aldrich (St. Louis, MO). Mass spectrometry grade trypsin was acquired from Promega (Madison, WI). Endoproteinase GluC and PNGase F with 10×G7 reaction buffer (0.5 M sodium phosphate) were purchased from New England Biolabs (Ipswich, MA).
Disodium phosphate na2hpo4
Manufactured by Mallinckrodt
Disodium phosphate (Na2HPO4) is a chemical compound that is commonly used as a laboratory reagent. It is a white, crystalline solid that is soluble in water. Disodium phosphate is a source of the phosphate ion (PO4^2-) and is often used in buffer solutions, as a pH regulator, and as a desiccant.
Automatically generated - may contain errors
Lab products found in correlation
Mass spectrometry grade trypsin, by Promega (2 mentions)
Hplc grade acetonitrile, by Thermo Fisher Scientific (2 mentions)
Sodium chloride (nacl), by Mallinckrodt (2 mentions)
Fetuin, by Merck Group (1 mentions)
Formic acid, by Thermo Fisher Scientific (1 mentions)
Endoproteinase gluc, by New England Biolabs (1 mentions)
Pngase f with 10 g7 reaction buffer 0.5 m sodium phosphate, by New England Biolabs (1 mentions)
Iodoacetamide iaa, by Merck Group (1 mentions)
Sodium metaperiodate, by Merck Group (1 mentions)
Aleuria aurantia lectin, by Vector Laboratories (1 mentions)
Sodium acetate naac, by Merck Group (1 mentions)
Sephadex g 25 spin column, by GE Healthcare (1 mentions)
Acetic acid, by Merck Group (1 mentions)
Pngase f, by New England Biolabs (1 mentions)
2 protocols using disodium phosphate na2hpo4
1
Glycoprotein Characterization by Mass Spectrometry
2
HPLC-based Glycopeptide Enrichment
Check if the same lab product or an alternative is used in the 5 most similar protocols
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HPLC-grade solvents, including methanol,
isopropanol, and water, were procured from Macron Fine Chemicals-Avantor
Performance Materials (Center Valley, PA). HPLC-grade acetonitrile
(ACN) was purchased from Fisher Scientific (Pittsburgh, PA). Sodium
chloride (NaCl) and disodium phosphate (Na2HPO4) were obtained from Mallinckrodt Chemicals (Phillipsburg, NJ). MS-grade
formic acid (FA), trifluoroacetic acid (TFA), and acetic acid were
obtained from Sigma-Aldrich (St. Louis, MO).d ,l -Dithiothreitol (DTT), iodoacetamide (IAA), 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate
(CHAPS), sodium dodecyl sulfate (SDS), urea, sodium meta-periodate,
and sodium acetate (NaAc) were also purchased from Sigma-Aldrich.
Sephadex G-25 spin column was obtained from GE Healthcare Life Science
(Pittsburgh, PA). Agarose-bound Sambucus nigra agglutinin
and Aleuria aurantia lectin were purchased from Vector
Laboratories (Burlingame, CA). 1 μm diameter BcMag hydrazide-modified
magnetic beads were acquired from Bioclone (San Diego, CA). Mass-spectrometry-grade
trypsin was obtained from Promega (Madison, WI), while PNGase F was
purchased from New England Biolabs (Ipswich, MA).
isopropanol, and water, were procured from Macron Fine Chemicals-Avantor
Performance Materials (Center Valley, PA). HPLC-grade acetonitrile
(ACN) was purchased from Fisher Scientific (Pittsburgh, PA). Sodium
chloride (NaCl) and disodium phosphate (Na2HPO4) were obtained from Mallinckrodt Chemicals (Phillipsburg, NJ). MS-grade
formic acid (FA), trifluoroacetic acid (TFA), and acetic acid were
obtained from Sigma-Aldrich (St. Louis, MO).
(CHAPS), sodium dodecyl sulfate (SDS), urea, sodium meta-periodate,
and sodium acetate (NaAc) were also purchased from Sigma-Aldrich.
Sephadex G-25 spin column was obtained from GE Healthcare Life Science
(Pittsburgh, PA). Agarose-bound Sambucus nigra agglutinin
and Aleuria aurantia lectin were purchased from Vector
Laboratories (Burlingame, CA). 1 μm diameter BcMag hydrazide-modified
magnetic beads were acquired from Bioclone (San Diego, CA). Mass-spectrometry-grade
trypsin was obtained from Promega (Madison, WI), while PNGase F was
purchased from New England Biolabs (Ipswich, MA).
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