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2 protocols using 2 ethyl 3 5 dimethyl pyrazine

1

Volatile Compound Extraction Protocols

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Diethyl ether (analytical grade reagent) was obtained from S.D. Fine Chem. Ltd., Mumbai, India and was double distilled before use. Sodium sulphate (Chemco fine chemicals, India), methional, hexanal, benzothiazole, decanal, p-vinyl guaiacol, dimethyl trisulfide and 2-ethyl-3,5-dimethyl pyrazine, 2,6-dimethyl pyrazine and benzaldehyde were purchased from Sigma-Aldrich, USA. Benzene acetaldehyde and 1-octen-3-ol were purchased from Fluka, USA. 3-methyl butanol was purchased from Reachim, Russia.
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2

Characterization of Maize Flour Flavor Compounds

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Hexanal, 2-methylpyrazine, 2,3-dimethylpyrazine, 2,5-dimethylpyrazine, 2-methyl-2-thiazoline, 2-pentylfuran, 2-ethyl-3,5-dimethylpyrazine, 3-hydroxy-2-methyl-4H-pyran-4-one, 2-methylphenol, 2-acetyl-2-thiazoline, (E,E)-2,4-decadienal, 2-methoxy-4-vinylphenol, 4-hydroxy-3-methoxybenzaldehyde, 4-heptanone, 2-methyl-3-heptanone, trisodium phosphate, calcium carbonate, anhydrous sodium sulfate, corn starch and sodium chloride were purchased from Sigma-Aldrich (St. Louis, MO, USA). Methanol and methylene chloride, in GC-Resolv® grade, were purchased from Fischer Scientific (Pittsburgh, PA, USA). Maizewise™ whole grain and Innovasure™ refined maize flours were purchased from Cargill (Minneapolis, MN, USA). All the sensory reference materials, dry uncooked Bob′s Red Mill Steel Cut Oats, Bergen Unsalted Lightly Roasted Almonds, Organic Valley Whole UHT Milk, Toasted Wonder Bread™, Nilla® Wafers, and Old Dutch Restaurante® Style Yellow Corn Tortillas Chips, were purchased from a local grocery store.
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