Target-specific shRNA was obtained in the pGIPZ lentiviral vector system (OpenBiosystems) from the MD Anderson shRNA and ORFeome Core Facility. Lentiviral packaging was performed in HEK-293T cells using the pCMV deltaR8.2 and pMD2.G vectors produced by the Didier Trono laboratory and made available through the Addgene repository. Target cells were infected with the virus-containing medium in the presence of 8 μg/mL polybrene.
Pgipz lentiviral vector system
Manufactured by Thermo Fisher Scientific
The PGIPZ lentiviral vector system is a tool used for gene expression and gene knockdown studies in mammalian cell lines. The system includes a lentiviral vector, packaging components, and a fluorescent reporter to facilitate monitoring of transduction and knockdown efficiency.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using pgipz lentiviral vector system
1
Lentiviral Transduction of Target Cells
2
Lentiviral Transduction of Target Cells
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