Ab204123

Manufactured by Abcam

Ab204123 is a laboratory equipment product. It is designed for general laboratory use. The core function of this product is to provide a specific capability within the laboratory setting. Detailed description of the product's features and intended use is not available.

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Lab products found in correlation

Ma5 11222, by Thermo Fisher Scientific (2 mentions) Alexa 488, by Thermo Fisher Scientific (1 mentions) Flag tag (flag), by Merck Group (1 mentions) Alexa 594, by Thermo Fisher Scientific (1 mentions) Goat anti rabbit igg hrp, by Bio-Rad (1 mentions) Ab6982, by Abcam (1 mentions) Ab1056, by Merck Group (1 mentions)

3 protocols using ab204123

Adenovirus Protein Extraction and Detection

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The proteins were extracted by lysis with RIPA buffer. Rabbit polyclonal antibody for the HAdV-5 (ab6982) and for E1A (ab204123) were obtained from Abcam. Monoclonal antibodies were purchased from: Flag (Sigma, F3165), Myc (Merck, MABE282), CAR (Cell Signaling, 5670S), and integrin Vα (Cell Signaling, 60896S). Secondary antibodies that were conjugated to Alexa488 (Invitrogen, A32723) and Alexa594 (Invitrogen, A32740) were obtained from Abcam. Goat anti-rabbit IgG-HRP and Goat anti-mouse IgG-HRP antibodies were obtained from Bio-Rad (cat. no. 170-6515 and 170-6516). Western blot and immunofluorescence staining were performed according to the standard protocols with the antibodies diluted, as recommended by manufacturers.

Dalidowska I., Gazi O., Sulejczak D., Przybylski M, & Bieganowski P. (2021). Heat Shock Protein 90 Chaperones E1A Early Protein of Adenovirus 5 and Is Essential for Replication of the Virus. International Journal of Molecular Sciences, 22(4), 2020.

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Immunostaining of Mouse Tumor Tissue

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Parafilm-imbedded sections of mouse tumor tissue were immunostained for antibodies specific to adenoviral goat-hexon (1:750; AB1056, Millipore), mouse-E1A (1:750; ab204123, Abcam), mouse-fiber (1:750; MA5-11222, Thermo), and rabbit-cleaved caspase 3 (1:200; Cell Signaling, 9661) following conventional procedures.

Nikrad J.A., Galvin R.T., Sheehy M.M., Novacek E.L., Jacobsen K.L., Corbière S.M., Beckmann P.J., Jubenville T.A., Yamamoto M, & Largaespada D.A. (2024). Conditionally replicative adenovirus as a therapy for malignant peripheral nerve sheath tumors. Molecular Therapy Oncology, 32(2), 200783.

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Adenovirus Protein Expression Analysis

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The cells were infected with RGD-COX2-CRAd at various MOIs (VP/cell). The samples were lysed 2 days post-infection and subjected to sodium dodecyl sulphate-Tris-glycine gel electrophoresis. The membranes were incubated with the following antibodies: E1A, (1:1,000, ab204123, Abcam), fiber (1:1,000, MA5-11222, Thermo), Hexon (1:5,000 b-12354), and GRB-2 (1:1,000; 610111, BD).

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