Anti mouse igg alexa fluor 488 conjugate

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Anti-mouse IgG-Alexa Fluor 488 conjugate is a fluorescently labeled secondary antibody used for the detection of mouse primary antibodies in various immunoassays and imaging applications. The Alexa Fluor 488 dye provides bright green fluorescence, enabling sensitive and specific detection of the target proteins.

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Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Dojindo Laboratories (1 mentions) Anti cd16 cd32 mab, by BioLegend (1 mentions) 5hh2 2a8, by Merck Group (1 mentions) Streptavidin alexa fluor 568 conjugate, by Thermo Fisher Scientific (1 mentions) Fv1200, by Olympus (1 mentions) Fv10 asw, by Olympus (1 mentions) Evos m5000, by Thermo Fisher Scientific (1 mentions) Sc 66020, by Santa Cruz Biotechnology (1 mentions) Fluoroshield with dapi, by Merck Group (1 mentions) Anti β actin, by Abcam (1 mentions) Anti mouse igg hrp conjugate, by Cell Signaling Technology (1 mentions) Anti mouse igg alexa fluor 594 conjugate, by Thermo Fisher Scientific (1 mentions) Anti ha tag c29f4, by Cell Signaling Technology (1 mentions) Anti rabbit igg ap conjugate, by Merck Group (1 mentions) Alexa fluor 568 phalloidin, by Thermo Fisher Scientific (1 mentions)

4 protocols using anti mouse igg alexa fluor 488 conjugate

Histone H2B Binding Assay in J774A.1 Cells

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J774A.1 cells, cultured on glass-based dishes, were preincubated with anti CD16/CD32 mAb (93, Biolegend) to block the FcγRII/III receptors and incubated with anti-histone H2B mAb (5HH2-2A8, Millipore, 1:200 dilution) and either 100 µg/ml Bt-BSA or Bt-AGEs at 4 °C for 15 min in HBSS-BSA. After washing three times with PBS (−), the cells were stained with streptavidin-Alexa Fluor 568 conjugate (1:1,000 dilution, Invitrogen) and anti-mouse IgG-Alexa Fluor 488 conjugate (1:1,000 dilution, Invitrogen) at 4 °C for 15 min in HBSS-BSA. After washing, the stained cells were fixed with 4% paraformaldehyde in PBS (−) for 10 min at room temperature and further stained with 1 µg/ml 4’,6-Diamidino-2-phenylindole (DAPI, Dojindo). Fluorescent images were obtained using a confocal microscope (FV1200, Olympus) and analyzed by Olympus software (FV10-ASW, Olympus).

Itakura M., Yamaguchi K., Kitazawa R., Lim S.Y., Anan Y., Yoshitake J., Shibata T., Negishi L., Sugawa H., Nagai R, & Uchida K. (2022). Histone functions as a cell-surface receptor for AGEs. Nature Communications, 13, 2974.

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Quantifying Epithelial Cell Markers in Lung Tissue

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For measurement of epithelial cell marker expression in the lung, it was stained with anti-EpCAM (sc-66020, Santa Cruz Biotechnology). The lung-embedded paraffin was sliced into 4 μm sections, and the paraffin was dissolved. It was boiled with Antigen Retrieval solution (0.1 M Sodium Citrate, pH 6) at 121 °C for 1 min. After washed, it reacted with peroxidase buffer (Peroxidase, Methanol). Additionally, then, it was blocked with 10% normal goat serum for 1 h at room temperature and incubated with antibody for overnight. A secondary antibody (anti-mouse IgG, Alexa Fluor 488 conjugate, #A32731, Invitrogen) was reacted for 1 h, and mounted by Fluoroshield with DAPI (#6057, Sigma-Aldrich, St. Louis, MO, USA). Slides were observed by microscope (EVOS M5000; Thermo Fisher Scientific, Waltham, MA, USA) under the same conditions. Relative intensity was measured by ImageJ (National Institutes of Health, Bethesda, MD, USA)

Park J., Jang J., Cha S.R., Baek H., Lee J., Hong S.H., Lee H.A., Lee T.J, & Yang S.R. (2022). L-carnosine Attenuates Bleomycin-Induced Oxidative Stress via NFκB Pathway in the Pathogenesis of Pulmonary Fibrosis. Antioxidants, 11(12), 2462.

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Antibody characterization in DNA damage response

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Anti-RTEL1, produced in Rabbit (Sigma, Cat. No. HPA067329); Anti-RPA1, produced in Mouse (Sigma, Cat. No. SAB1406399); Anti-RPA 32 kDa subunit (9H8), produced in Mouse (Santa Cruz Biotechnology, Cat. No. sc-56770); Anti-HA tag (C29F4), produced in Rabbit (Cell Signalling Technology, Cat. No. 3724); Anti-gamma H2A.X (phospho S139) antibody [3F2], produced in mouse (Abcam, Cat. No. ab22551); Anti-beta Actin, produced in Mouse (Abcam, Cat. No. ab8226); Anti-Rabbit IgG, HRP-conjugate (Millipore, Cat. No. 12–348); Anti-Mouse IgG, HRP-conjugate (Cell Signalling Technology, Cat. No. 7076); Anti-Rabbit IgG, Alexa Fluor™ 488-conjugate (Invitrogen, Cat. No. A-11008); Anti-Rabbit IgG, Alexa Fluor™ 594-conjugate (Invitrogen, Cat. No. SA5-10040); Anti-Mouse IgG, Alexa Fluor™ 488-conjugate (Invitrogen, Cat. No. A-11001); Anti-Mouse IgG, Alexa Fluor™ 594-conjugate (Invitrogen, Cat. No. A-11032)

Kumar N., Taneja A., Ghosh M., Rothweiler U., Sundaresan N.R, & Singh M. (2023). Harmonin homology domain-mediated interaction of RTEL1 helicase with RPA and DNA provides insights into its recruitment to DNA repair sites. Nucleic Acids Research, 52(3), 1450-1470.

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Characterization of SARS-CoV-2 Entry Factors

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The following antibodies were used: rabbit polyclonal anti-ACE2 (#SAB2100025), anti-mouse polyclonal Immunoglobulins (IgG, IgA, IgM) alkaline phosphatase (AP)-conjugate and anti-rabbit IgG AP-conjugate were purchased from Sigma-Aldrich (St. Louis, MO, USA); rabbit polyclonal anti-TMPRSS2 (#PA5-116054), rabbit monoclonal anti-CD147 (#MA5-29060), anti-mouse IgG Alexa Fluor^® 488-conjugate, Alexa Fluor^® 568 phalloidin (#A12380), and V5 Tag Monoclonal Antibody Alexa Fluor 488-conjugate (#37-7500-A488) were bought from Invitrogen; SARS-CoV/SARS-CoV-2 (COVID-19) spike antibody (#GTX632604) was purchased from GeneTex, while anti-SARS/SARS-CoV-2 Coronavirus Nucleocapsid Antibody (#MA1-7403) was from Thermo Fisher Scientific. SARS-CoV-2 Spike protein S1 (aa 1-674) Recombinant antigen was bought from Merck Millipore (Darmstadt, Germania). All other chemicals were purchased from Sigma-Aldrich.

Agostinis C., Toffoli M., Spazzapan M., Balduit A., Zito G., Mangogna A., Zupin L., Salviato T., Maiocchi S., Romano F., Crovella S., Fontana F., Braga L., Confalonieri M., Ricci G., Kishore U, & Bulla R. (2022). SARS-CoV-2 modulates virus receptor expression in placenta and can induce trophoblast fusion, inflammation and endothelial permeability. Frontiers in Immunology, 13, 957224.

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