Oci aml2

Myelodysplastic SKM-1 cells and human myeloid leukemia OCI-AML2, SH-1, KU812, MEG01, and K562 cells were purchased from the Shanghai Cell Bank, Chinese Academy of Sciences. SKM-1 cells were cultured in DMEM high-glucose medium (Gibco), and OCI-AML2, SH-1, HL-60, MEG01, and K562 cells were cultured in IMDM medium (Gibco). All media were supplemented with 10% fetal bovine serum (Biological Industries) and 1% cyanin-streptomycin (Biosharp). All cells were cultured at 37.5°C in a 5% CO₂ incubator.

HL-60 (acute promyelocytic leukemia cell line), THP-1 (acute monocytic leukemia cell line), OCI-AML2 (acute myelomonocytic leukemia cell line) and OCI-AML3 (acute myelomonocytic leukemia cell line) cell lines were purchased from Shanghai Cell Bank of the Chinese Academy of Sciences. KASUMI-1 (acute myeloblastic leukemia cell line) cell line was gifted by Professor Chen Saijuan (Shanghai Institute of Hematology, Shanghai, China). These cells were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) (Gibco) at 37 °C in a humidified incubator containing 5% CO2. MV4-11(acute myelomonocytic leukemia cell line) and MOLM-13(acute monocytic leukemia cell line) cell lines were a kind gift from Professor Ravi Bhatia (City of Hope National Medical Center, Duarte, CA, USA). These two cell lines were cultured in Iscove's Modified Dulbecco's Medium (IMDM) supplemented with 10% (FBS). Diagnostic AML patient samples were purified by standard Ficoll‐Hypaque (Sigma-Aldrich) density centrifugation, then cultured in RPMI 1640 with 10% FBS.