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6 protocols using acrylic acid

1

Hydrogel Synthesis Using FeCl3

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Iron chloride (FeCl3; Merck, Rahway, NJ, USA), sodium chloride (NaCl; Samchun Chemicals, Seoul, Republic of Korea), acrylic acid (AA, >99%; Tokyo Chemical Industry, Tokyo, Japan), poly(ethylene glycol) diacrylate (PEGDA, Mn∼250; Merck, Rahway, NJ, USA), and ammonium persulfate (APS; Samchun Chemicals, Seoul, Republic of Korea) were purchased and used without further purification.
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2

Graphite-Based Thermal Conductive Adhesive Synthesis

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Butyl acrylate (>99%), 2-ethylhexyl acrylate (>99%), ethyl acrylate (>99%), 2-hydroxyethyl acrylate (>95%), acrylic acid (>99%), abietic acid (>80%), triethylamine (>99%), and 2-isocyanatoethyl methacrylate (>98%) were purchased from Tokyo Chemical Industry. Tris(4-hydroxyphenyl)methane triglycidyl ether (>95%) and dibutyltin dilaurate (95%) were purchased from Sigma-Aldrich Korea Ltd. (Yongin, Korea). Toluene (99.5%), ethyl acetate (99.5%), and methyl ethyl ketone (99.5%) were purchased from Samchun Chemical (Pyeongtaek, Korea). Azobisisobutyronitrile (AIBN, 98%) was purchased from Junsei Chemical (Tokyo, Japan). All chemicals were used as received without further purification. The epoxy crosslinker was used after dilution to a solid content of 5 wt% with Toluene. A 17-μm-thick graphite sheet was purchased from Tanyuan technology (Changzhou, China). A silicone-coated polyethylene terephthalate (PET) film and a polyethylene terephthalate (PET) film were purchased from SKC (Seoul, Korea). A stainless steel (SS) plate was purchased from MMSTECH (Bucheon, Korea). The micro structure of the g-TC film was analyzed using a scanning electron microscope (SEM, JSM 6380, JEOL, Tokyo, Japan) and a digital microscope (MXB-5000REZ, HIROX, Tokyo, Japan).
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3

Thermoresponsive Drug Delivery Hydrogels

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The
following materials were obtained from
commercial sources: N-isopropylacrylamide (NIPAm,
≥98.0% purity), acrylic acid (AAc, ≥99.0% purity), and N-phenylacrylamide (PAA, ≥98.0% purity) from Tokyo
Chemical Industry Co., Ltd. (Tokyo, Japan); ammonium persulfate (APS,
≥98.0%, purity) and melittin (from honey bee, ≥85.0%)
from Sigma-Aldrich, Inc. (St Louis, MO, USA); N,N′-methylenebis(acrylamide) (bis, ≥97.0%,
purity), N-tert-butylacrylamide
(TBAm, ≥95.0%, purity), sodium dodecyl sulfate (SDS, ≥95.0%,
purity), PTX (≥98.0%, purity), and isoflurane (≥98.0%
purity) from Wako Pure Chemical Industries, Ltd. (Osaka, Japan); N- Taxol, [3H] from Moravek Biochemicals, Inc.
(Brea, CA, USA); and Hionic Fluor from PerkinElmer Japan Co. Ltd.
(Yokohama, Kanagawa, Japan). NIPAm was recrystallized from hexane
(Wako Pure Chemical Industries, Ltd., 96.0%) before polymerization.
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4

Monolayer Formation with Thiol Compounds

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16-Amino-1-hexadecanethiol hydrochloride was purchased from Dojindo Laboratories (Japan). Acrylic acid, tetra(ethylene glycol)diacrylate, and 2-oxoglutaric acid were purchased from Tokyo Chemical Industry Co., Ltd. (Japan). Acrylic acid was used after purification by vacuum distillation. Hexa(ethylene glycol)undecanethiol was synthesized according to our previous report.50 (link) NaCl was purchased from FUJIFILM Wako Pure Chemical Corp. (Japan). 1-Methylimidazolium hydrogen sulfate was purchased from Sigma-Aldrich Co. LLC. (USA).
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5

Synthesis and Characterization of Biomimetic Hydrogels

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Styrene, l-cysteine hydrochloride, 2-hydroxyethyl methacrylate (HEMA), N,N′-methylenebisacrylamide (BIS) and diethoxyacetophenone (DEAP) were purchased from J&K Scientific Ltd. (China). 1-Propanol, sodium dodecyl sulfate (SDS), 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB), acetylthiocholine iodide (ATChI), Coomassie Brilliant Blue (G-250), dichlorvos, malathion, methidathion, acephate, glufosinate-ammonium, and Dipterex were purchased from Aladdin Co., Ltd. (China). Acrylic acid (AA) was purchased from Tokyo Chemical Industry Co., Ltd. (Japan). H2SO4 (95–98 wt%), phosphoric acid, HCl, NaOH, K2S2O8 and dimethyl sulfoxide (DMSO) were purchased from Beijing Chemical Plants (China). Tris(hydroxymethyl)aminomethane (Tris) and acetylcholinesterase (AChE) were obtained from Sigma (USA). Acrylamide (AM) was purchased from Amresco (USA). H2O2 (30 wt%) was purchased from Tianjin Fuyu Fine Chemical Co., Ltd. (China). 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) was supplied by Shanghai Medpep Co., Ltd. (China). The inhibitor in Styrene needed to be removed through an Al2O3 column before use, whereas the other materials were used as received. Deionized water was produced by the Puris-Evo CB Water System.
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6

Synthesis and Characterization of Acrylic Monomers

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2-Hydroxethyl acrylate (HEA) 96%, 2,2′-azobis(2-methylpropionitrile) (AIBN) 98%, and 2-hydroxyethyl methacrylate (HEMA) were all obtained from Sigma-Aldrich (St. Louis, MO, USA). Tetraethylene glycol dimethacrylate (TTEGDMA-pure grade with MEHQ) and urethane dimethacrylate (UDMA) were both purchased from Esstech Inc. (Essington, PA, USA), whereas acrylic acid (AA) > 99.0% was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). 2-mercaptoethanol (ME) 99% was purchased from Acros Organics (Fair Lawn, NJ, USA). Ebecryl 270™ (EB270) and Genocure* LTM were kindly donated by Allnex (Alpharetta, GA, USA) and RAHN USA Corp. (Aurora, IL, USA), respectively, and all solvents used were obtained from Fisher.
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