Mab13

Manufactured by BD

MAb13 is a laboratory equipment product. It is a monoclonal antibody used for research applications. The core function of MAb13 is to detect and bind to specific target analytes in biological samples.

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Lab products found in correlation

Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Anti α tubulin, by Santa Cruz Biotechnology (1 mentions) Megm complete medium, by Lonza (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Anti gapdh, by Merck Group (1 mentions) Rat igg2a, by Thermo Fisher Scientific (1 mentions) Mouse igg1, by Agilent Technologies (1 mentions) Mouse igg2a, by Thermo Fisher Scientific (1 mentions) Cy 90, by Merck Group (1 mentions) Sb216763 s3442, by Merck Group (1 mentions)

3 protocols using mab13

Cell Culture and Antibody Immunostaining

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U2Os, U2Os-AP2-GFP, U2Os-AP2-halo, U2Os-AP2-GFP-ITGB5-mScarlet, HeLa-AP2-GFP, and HeLa-AP2-halo were cultured in MEM supplemented with 10% FBS (Gibco) and penicillin–streptomycin (100 U/ml, Thermo Fisher Scientific). HDF-AP2-halo and Caco2-AP2-halo were cultured in DMEM supplemented with 10% FBS (Gibco) and penicillin–streptomycin (100 U/ml, Thermo Fisher Scientific). MCF7-AP2-halo was cultured in DMEM supplemented with 10% FBS (Gibco) and penicillin–streptomycin (100 U/ml, Thermo Fisher Scientific), 2 mM glutamine, 5 µg/ml human insulin, and 1 µM sodium pyruvate. hMEC-AP2-GFP was cultured in MEGM complete medium (Lonza).
The following primary antibodies were used: anti-human integrin β1 clones 12G10 (NB100-63255; Novus bio), mAb13 (552828; BD), total integrin β1 (MAB2252; Millipore), anti-human integrin αvβ5 clone 15F11 (MAB2019Z; Millipore), anti-human integrin α5 clone SNAKA51 (AF1846; R&D), anti-human Tensin1 (SAB4200283; Sigma-Aldrich), anti-human p-paxillin Y118 (69363; Cell Signaling), anti-human-Talin1 (T3287; Sigma-Aldrich), anti-α-tubulin (sc-32293; SantaCruz Biotechnology), and anti-GAPDH (G9545; Sigma-Aldrich). Corresponding secondary antibodies raised against rabbit or mouse IgG were purchased from Jackson Immunoresearch.

Hakanpää L., Abouelezz A., Lenaerts A.S., Culfa S., Algie M., Bärlund J., Katajisto P., McMahon H, & Almeida-Souza L. (2023). Reticular adhesions are assembled at flat clathrin lattices and opposed by active integrin α5β1. The Journal of Cell Biology, 222(8), e202303107.

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Integrin Function Blocking Assays

Cited 2 times

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The following antibodies were used at 10μg/ml to block integrin function: rat IgG2a anti-human CD29/β1-integrin (Mab13; BD Pharmingen); mouse IgG2a anti-human CD18/β2-integrin (IB4; Calbiochem); mouse IgG1 anti-human CD61/β3-integrin (SZ21; Beckman Coulter); matched control antibodies, rat IgG2a, mouse IgG2a (both eBioscience), mouse IgG1 (DAKO). In some experiments, RGDS peptide (Arg-Gly-Asp-Ser, 0.5 mM; Sigma) or CT7010 (20 μM) was used instead of antibody. CT7010 is a low molecular weight, non-peptide, inhibitor of β₂-integrin function (gift of Dr. Tony Shock, Celltech R&D) The above antibodies and inhibitors have previously been shown to block functions [11 (link),40 (link),41 (link)].
Neutrophils were treated with antibodies for 10 min prior to addition to the gels. For endothelial monolayers, after 10 min the non-adherent cells were removed by washing with M199/BSA (0.15%) and 1 ml of M199 BSA (0.15%) containing the agent was re-added to the gel. In separate experiments, agents were added after 15min when initial migration had occurred. We showed that these added antibodies did reach and bind to integrins within 1h by retrieving cells, labelling them with secondary anti-integrin and flow cytometry (see below). Neutrophils were also treated with antibodies 10min before analysis in the phagocytosis assays.

Luo D., McGettrick H.M., Stone P.C., Rainger G.E, & Nash G.B. (2015). The Roles of Integrins in Function of Human Neutrophils after Their Migration through Endothelium into Interstitial Matrix. PLoS ONE, 10(2), e0118593.

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Antibody Detection of Integrin Variants

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Primary antibodies used for the detection of the α6A and α6B variants were anti-α6A [western blot (WB): 1/500, immunofluorescence (IF): 1/100] (1A10, Millipore, Etobicoke, Ontario) and anti-α6B (WB: 1/500, IF: 1/100) (6B4, Millipore). These antibodies were originally a generous gift from Dr A.Sonnenberg (Division of Cell Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands). Other primary antibodies used were anti-integrin β4 (WB: 1/5000, IF: 1/100) (3E1, Millipore), anti-integrin α6 (IF: 1/1000) (GOH3, Millipore), anti-β-actin (WB: 1/75 000) (C4, Millipore), anti-active-β-catenin (WB: 1/2500) (8E7, Millipore) recognizing the dephosphorylated form of β-catenin on Ser37 and Thr41 (sites of GSK3β phosphorylation), anti β-catenin (WB: 1/2500) (610153, BD Biosciences, Mississauga, Ontario), anti-DVL2 (WB: 1/2500) (30D2, Cell Signaling Technology, Danvers, MA), anti-cytokeratin 18 (WB: 1/1 000 000) (CY-90, Sigma–Aldrich, Oakville, Ontario), anti-histone H1 (WB: 1/1000) (AE-4, Santa Cruz Biotechnologies, Santa Cruz, CA), anti-integrin β1 (WB: 1/1000) (Mab13, BD Biosciences), anti-GSK3β (WB: 1/5000) (27C10, Cell Signaling Technology) and anti-H3K27me3 (WB: 1/1000) (07-449, Millipore). The pharmacological inhibitor of GSK3β (SB216763, S3442, Sigma–Aldrich) was used at a final concentration of 20 µM. The protease inhibitor cocktail (P8340) was purchased from Sigma.

Groulx J.F., Giroux V., Beauséjour M., Boudjadi S., Basora N., Carrier J.C, & Beaulieu J.F. (2014). Integrin α6A splice variant regulates proliferation and the Wnt/β-catenin pathway in human colorectal cancer cells. Carcinogenesis, 35(6), 1217-1227.

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