Anti fab dylight 488

Anti-FAK, anti-ALCAM-PE, anti-ICAM1-PE, anti-VCAM1-PE, anti-VE cadherin, anti-von Willebrand (vWF) factor, anti-CHS1 were purchased from Abcam (Cambridge, MA, USA). OX124, mouse anti-human monoclonal antibody against HS, was a kind gift from Dr. Marion Brown, Oxford, UK. Biotin-labeled Anti-CD18 KIM127 was purchased from Exploratory Research Cell Tech Therapeutics Ltd. (Slough, UK). Anti-phospho-SLP-76; pTyr128, anti-VE-cadherin were purchased from Cell signaling Technologies (Danvers, MA, USA). Anti-HIF-1, anti-pZAP-70-FITC, anti-pZAP-70-PE, anti- LAG3, anti-TIM3, anti-PD-1, eFluor 670, anti-CD45-APC, anti-CHS-PerCP, anti-CCR7, anti-CD45RO were purchased from BD biosciences (Franklin Lakes, NJ, USA). Anti-Talin-1 and anti-Vinculin were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Goat anti-human Fc-PE was purchased from Millipore (Billerica, MA, USA). Alexa Fluor (AF) labelled secondary anti-mouse, anti-goat anti-rabbit antibodies, streptavidin AF 488 antibody; Texas Red Phalloidin and AF488 Phalloidin were purchased from Life Technologies (Carlsbad, CA, USA). Anti-Fab DyLight 488 was purchased from Jackson ImmunoResearch (Suffolk, UK)