Cells were fixed with 2% paraformaldehyde and 2% sucrose in 1× PBS (pH 7.6) for 10min and then permeabilized with 1% NP-40. After pre-incubation with 5% BSA/PBS, cells were incubated first with the primary antibody and then with the secondary antibody in 1% BSA/PBS for 1h at room temperature. After DAPI staining, the slides were mounted with ProLong mounting medium (Invitrogen, CA, USA). Fluorescence microscopy was performed using a Nikon Eclipse 90i fluorescence microscope and a Nikon Digital Sight CCD (controlled by NIS-Elements 3.0 software).
Digital sight ccd
Manufactured by Nikon
The Digital Sight CCD is a high-performance charge-coupled device (CCD) camera designed for laboratory and scientific applications. It provides precise image capture and data acquisition capabilities for a wide range of microscopy and imaging tasks.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using digital sight ccd
1
Immunofluorescence Visualization of Cellular Proteins
2
Multimodal Characterization of Nanomaterials
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Optical images were obtained using a Nikon Eclipse Ti-U inverted microscope fitted with a Nikon Digital Sight CCD. SEM images were collected using a Hitachi S-4800 field-emission microscope operating at 15 kV. Raman spectra and mappings were performed with the Renishaw inVia Raman microscope and Nanophoton Raman 11 microscope. Bruker Dimension FastScan was used for AFM and KPFM characterization. KPFM characterization was carried out using a doped silicon tip (PFQNE-AL, Bruker) with a probe radius of approximately 5 nm and a spring constant of around 0.8 N m -1 under peak force mode, with a 60 nm lift scan height. TEM and STEM images, and EDS maps were acquired using an aberration-corrected JEM-ARM300F microscope operating at 300 kV, located at the Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing. Cross-sectional samples for TEM were fabricated using an FEI Helios-600i focused ion beam (FIB).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!