RNA libraries were prepared at the Iowa Institute of Human Genetics (IIHG), Genomics Division, using the Illumina TruSeq Stranded Total RNA with Ribo-Zero gold sample preparation kit (Illumina, Inc., San Diego, CA). Library concentrations were measured using KAPA Illumina Library Quantification Kit (KAPA Biosystems, Wilmington, MA). Polled libraries were sequenced on Illumina NovaSeq6000 sequencer with 150-bp Paired-End chemistry (Illumina) at the IIHG core.
150 bp paired end chemistry
Manufactured by Illumina
The 150-bp Paired-End chemistry is a laboratory equipment product designed for DNA sequencing applications. It enables the generation of paired-end reads with a read length of 150 base pairs. The core function of this product is to provide high-quality, accurate DNA sequence data for various research and analysis purposes.
Automatically generated - may contain errors
3 protocols using 150 bp paired end chemistry
1
RNA Sequencing Library Preparation
2
Transcriptome Sequencing Using Illumina NovaSeq
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RNA libraries were prepared at the Iowa Institute of Human Genetics (IIHG), Genomics Division, using the Illumina TruSeq Stranded Total RNA with Ribo-Zero gold sample preparation kit (Illumina, Inc., San Diego, CA). Library concentrations were measured using KAPA Illumina Library Quantification Kit (KAPA Biosystems, Wilmington, MA). Pooled libraries were sequenced on Illumina NovaSeq6000 sequencer with 150-bp Paired-End chemistry (Illumina) at the IIHG core.
3
RNA Sequencing of Hippocampal Transcripts
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RNA libraries from homecage and MWM mice were prepared at the Iowa Institute of Human Genetics (IIHG), Genomics Division, using the Illumina TruSeq Stranded Total RNA with Ribo-Zero gold sample preparation kit (Illumina, Inc., San Diego, CA). Library concentrations were measured with KAPA Illumina Library Quantification Kit (KAPA Biosystems, Wilmington, MA). Pooled libraries were sequenced on Illumina HiSeq 4000 sequencers with 150-bp Paired-End chemistry (Illumina) at the IIHG core.
The RNA seq data have been deposited in NCBI’s Gene Expression Omnibus and are accessible through GEO Series accession number GSE151681 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE151681 ). The code for analyses and figures related to RNA-seq data can be accessed through GitHub (github.com/ethanbahl/chatterjee2020_cbpkix ).
The RNA seq data have been deposited in NCBI’s Gene Expression Omnibus and are accessible through GEO Series accession number GSE151681 (
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