Myo10

MYO10 (Novus Biologicals, NBP1-87748) was used at 1:2000. JAG1 (Cell Signaling Technology, 70109) was used at 1:2000. IL13RA2 (Abcam, ab55275) was used at 1:2000. Cleaved Notch1 (Val1744) (Cell Signaling Technology, 4147) was used at 1:2000. α-Tubulin (Millipore, MAB1864) was used at 1:5000. Actin (Sigma, A2066) was used at 1:5000. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Cell Signaling Technology, 2118) was used at 1:5000. Peroxidase AffiniPure Goat Anti-Mouse immunoglobulin G (IgG) (H+L) (Jackson ImmunoResearch, 115-035-003) was used at 1:10,000. Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) (Jackson ImmunoResearch, 111-035-144) was used at 1:10,000. Peroxidase AffiniPure Goat Anti-Rat IgG (H+L) (Jackson ImmunoResearch, 112-035-003) was used at 1:10,000.

MYO10 (Novus Biologicals, NBP1-87748) was used at 1:1000. JAG1 (Cell Signaling Technology, 70109) was used at 1:1000. FN1 (Abcam, ab6328) was used at 1:1000. 4′,6-Diamidine-2′-phenylindole dihydrochloride (Sigma, D9542) was used as a nuclear counterstain at 300 nM. Phalloidin-488 (Thermo Fisher Scientific, A12379) or phalloidin-635 (Thermo Fisher Scientific, A34054) was dissolved in methanol and used at 1:100. Rhodamine-FN (Cytoskeleton, FNR01) was used at 1:50 and mixed with rBM. JAG1 antibody used for blocking (Sino Biological, 1164-MMO3) was used at 100 ng/ml.